Resource Project

资源项目

• 批准号: 9884805
• 负责人: Douglas Gordon Howe
• 金额: $ 133.87万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9884805
• 关键词: Affect; Animal Model; Antisense Oligonucleotides; Base Sequence; Behavior; Biomedical Research; Code; Communities; Data; Data Set; Databases; Development; Elements; Gene Expression; Generations; Genes; Genetic; Genetic Models; Genome; Genomics; Goals; Human; Human Biology; Human Genome; Individual; Institutes; Laboratories; Link; Modeling; Mutate; Mutation; Nomenclature; Organism; Organogenesis; Orthologous Gene; Other Genetics; Pattern; Phenotype; Physiological; Physiology; Play; Process; Proteins; Publications; Recovery; Research; Research Personnel; Resources; Role; Site; Techniques; Technology; Transgenic Organisms; Vertebrate Biology; Vertebrates; Work; Zebrafish; data exchange; gene function; genetic analysis; genome annotation; genome editing; human disease; human model; improved; insight; knock-down; knockout gene; member; model organisms databases; mouse genome; reference genome; tool; web site; wiki; zebrafish genome

PROJECT SUMMARY/ABSTRACT Resource Project Discovering the functions of the tens of thousands of genes in the human genome is a required step for understanding human biology and disease. Genetic model organisms, including zebrafish, play a critical role in this discovery process, because genetic analysis can connect gene sequence and function. Model organism databases, like ZFIN, provide tools required to make this connection. The zebrafish has emerged as a premier organism to study vertebrate biology. Powerful techniques allow rapid efficient generation and recovery of mutations affecting genes that orchestrate developmental patterning, organogenesis, physiology, and behavior. It is easy to study gene function by generating transgenic zebrafish, by knocking down gene function with morpholino antisense oligonucleotides, or by altering gene function by genome editing. The genome has been sequenced and about 50% of the protein coding genes have been mutated by targeted gene knockout technology. Large-scale projects are underway or planned that will produce functional data about almost all the genes and sequence-based functional elements in the genome. Multiple mutations and gene knockdowns can be combined in the same individual to study gene modifiers and other genetic interactions. The functions of most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene functions in other vertebrates, including humans. The long term goals for ZFIN are a) to be the community database resource for the laboratory use of zebrafish, b) to develop and support integrated zebrafish genetic, genomic, developmental, and physiological information, c) to maintain the definitive reference data sets of zebrafish research information, d) to link this information extensively to corresponding data in other model organism and human databases, e) to facilitate the use of zebrafish as a model for human biology, and f) to help serve the broad needs of the biomedical research community. In this part of the project, we will curate and integrate zebrafish research data into the ZFIN database and link them to human biology and disease, and we will develop maintain the zebrafish reference genome and provide ongoing annotation. We will also continue to improve zebrafish gene models.

项目概要/摘要资源项目 发现人类基因组中成千上万个基因的功能是人类基因组研究的必要步骤。 了解人类生物学和疾病。遗传模式生物，包括斑马鱼， 在这一发现过程中的关键作用，因为遗传分析可以连接基因序列， 功能模式生物数据库，如ZFIN，提供了建立这种联系所需的工具。 斑马鱼已经成为研究脊椎动物生物学的首要生物。强大 技术允许快速有效地产生和恢复影响基因的突变， 发育模式、器官发生、生理学和行为。研究基因功能很容易 通过产生转基因斑马鱼，通过用吗啉代反义核酸敲低基因功能， 通过寡核苷酸，或通过基因组编辑改变基因功能。基因组已经测序 通过靶向基因敲除技术，约50%的蛋白质编码基因发生突变。 正在进行或计划进行的大型项目将产生几乎所有 基因和基因组中基于序列的功能元件。多种突变和基因 敲除可以在同一个体中组合，以研究基因修饰剂和其他遗传修饰剂。 交互.这些基因中的大多数的功能在脊椎动物群体中是保守的。因此，在本发明中， 斑马鱼突变的分析提供了对其他脊椎动物基因功能的深入了解，包括 人类 ZFIN的长期目标是a）成为实验室使用的社区数据库资源 B）开发和支持整合的斑马鱼遗传、基因组、发育和 生理信息，c）维护斑马鱼研究的权威参考数据集 信息，d）将该信息广泛地与其他模式生物中的相应数据联系起来， 人类数据库，e）促进斑马鱼作为人类生物学模型的使用，以及f）帮助 满足生物医学研究界的广泛需求。 在项目的这一部分，我们将管理和整合斑马鱼的研究数据到ZFIN 数据库，并将它们与人类生物学和疾病联系起来，我们将开发和维护斑马鱼 参考基因组并提供持续注释。我们还将继续改进斑马鱼的基因 模型