Resource Project

项目摘要/摘要资源项目 发现人类基因组中数以万计的基因的功能是 了解人类生物学和疾病。遗传模式生物，包括斑马鱼，扮演着 在这一发现过程中起着关键作用，因为遗传分析可以将基因序列和 功能。像ZFIN这样的生物模型数据库提供了建立这种联系所需的工具。 斑马鱼已经成为研究脊椎动物生物学的首要生物。功能强大 技术允许快速有效地产生和恢复影响协调的基因的突变 发育模式、器官发生、生理和行为。研究基因的功能很容易 通过产生转基因斑马鱼，通过用吗啡反义基因击倒基因功能 寡核苷酸，或通过基因组编辑改变基因功能。基因组已经被测序 通过靶向基因敲除技术，大约50%的蛋白质编码基因发生了突变。 正在进行或计划中的大型项目将产生关于几乎所有 基因组中的基因和基于序列的功能元件。多重突变和基因 基因敲除可以在同一个体中结合起来研究基因修饰物和其他基因 互动。这些基因中的大多数功能在脊椎动物群体中是保守的。因此， 对斑马鱼突变的分析提供了对其他脊椎动物基因功能的洞察，包括 人类。 ZFIN的长期目标是a)成为供实验室使用的社区数据库资源 斑马鱼，b)开发和支持整合的斑马鱼遗传、基因组、发育和 生理信息，c)维护斑马鱼研究的明确参考数据集 信息，d)将该信息广泛地链接到其他模式生物体中的相应数据，以及 人类数据库，e)促进使用斑马鱼作为人类生物学模型，以及f)帮助 服务于生物医学研究界的广泛需求。 在项目的这一部分，我们将整理斑马鱼研究数据并将其整合到ZFIN中 数据库，并将它们与人类生物学和疾病联系起来，我们将开发维护斑马鱼 参考基因组并提供持续的注释。我们还将继续改进斑马鱼的基因 模特们。

PROJECT SUMMARY/ABSTRACT Resource Project Discovering the functions of the tens of thousands of genes in the human genome is a required step for understanding human biology and disease. Genetic model organisms, including zebrafish, play a critical role in this discovery process, because genetic analysis can connect gene sequence and function. Model organism databases, like ZFIN, provide tools required to make this connection. The zebrafish has emerged as a premier organism to study vertebrate biology. Powerful techniques allow rapid efficient generation and recovery of mutations affecting genes that orchestrate developmental patterning, organogenesis, physiology, and behavior. It is easy to study gene function by generating transgenic zebrafish, by knocking down gene function with morpholino antisense oligonucleotides, or by altering gene function by genome editing. The genome has been sequenced and about 50% of the protein coding genes have been mutated by targeted gene knockout technology. Large-scale projects are underway or planned that will produce functional data about almost all the genes and sequence-based functional elements in the genome. Multiple mutations and gene knockdowns can be combined in the same individual to study gene modifiers and other genetic interactions. The functions of most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene functions in other vertebrates, including humans. The long term goals for ZFIN are a) to be the community database resource for the laboratory use of zebrafish, b) to develop and support integrated zebrafish genetic, genomic, developmental, and physiological information, c) to maintain the definitive reference data sets of zebrafish research information, d) to link this information extensively to corresponding data in other model organism and human databases, e) to facilitate the use of zebrafish as a model for human biology, and f) to help serve the broad needs of the biomedical research community. In this part of the project, we will curate and integrate zebrafish research data into the ZFIN database and link them to human biology and disease, and we will develop maintain the zebrafish reference genome and provide ongoing annotation. We will also continue to improve zebrafish gene models.