Modelling CHO clonal phenotype variance for optimal bioprocessing platform design

建模 CHO 克隆表型方差以实现最佳生物加工平台设计

• 批准号: 2120380
• 金额: --
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2120380
• 关键词: Modelling; CHO; clonal; phenotype; variance

The project will use advanced screening, genome editing (e.g. CRISPR/Cas9)/synthetic biology and analytical technologies, to further develop an understanding of the cellular processes that limit and underpin cell metabolism, cell growth, recombinant protein product yield and quality. In turn, this understanding will be utilized to develop new CHO cell chassis and bioprocesses that enhance GSKs ability to rapidly generate cell lines making novel molecules at appropriate amounts and quality for investigation and progression towards clinical studies.

该项目将使用先进的筛选、基因组编辑（如CRISPR/Cas9）/合成生物学和分析技术，进一步了解限制和支撑细胞代谢、细胞生长、重组蛋白产品产量和质量的细胞过程。反过来，这种理解将用于开发新的CHO细胞底盘和生物工艺，以增强gsk快速生成细胞系的能力，以适当的数量和质量制造新分子，用于临床研究的调查和进展。