Diamond and Hydrogenated Carbon Microelectrodes

金刚石和氢化碳微电极

• 批准号: 6631452
• 负责人: GREG M SWAIN
• 金额: $ 11.21万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6631452
• 关键词: Raman spectrometry; X ray spectrometry; atomic force microscopy; bioassay; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biotechnology; capillary electrophoresis; carbon; chemical kinetics; electrical measurement; electrochemistry; hydrogen; liquid chromatography; mass spectrometry; microelectrodes; neurotransmitters; oxidation reduction reaction; scanning electron microscopy; surface coating; surface property

DESCRIPTION (provided by applicant): The proposed research will fabricate, evaluate and develop diamond and hydrogenated carbon fiber microelectrodes for use in the measurement and detection of electroactive neurotransmitters, related metabolites and other bioanalytes. These new, low oxide and chemically stable microelectrodes have the potential to significantly impact in vitro and in vivo neuroelectrochemical measurements because they solve several problems/complications often encountered with common sp2 carbon fiber microelectrodes. These problems/complications exist because of the presence of electroactive and ionizable surface carbon-oxygen functionalities. Boron-doped microcrystalline and nitrogen-incorporated nanocrystalline diamond films conformally deposited on 20-100 micron diameter W and Mo fibers, and hydrogen plasma treated carbon fibers (pitch and pan-based, 10-40 micron diameter) will be the microelectrodes investigated. These electrode materials offer advantages compared to commonly used sp2 carbon electrodes including a lower voltammetric and amperometric background current; no background voltammetnc features associated with electroactive surface carbon-oxygen functionalities; no variations in the response sensitivity for charged solution analytes due to the absence of ionizable carbon-oxygen functionalities; resistance to fouling due to the nonpolar, hydrogen-terminated surface; low limits of detection; and improved response precision and stability.The goals of the research are (i) to prepare and comprehensively characterize the microelectrodes, (ii) to demonstrate their efficacy for the measurement of neurotransmitters, metabolites and bioanalytes, (iii) to verify that the low oxide surfaces solve the problems/complications that exist in fast scan voltammetric measurements when oxygen functionalized electrodes are used, (iv) to develop a detailed understanding of the electrode reaction kinetics and mechanisms at the hydrogen-terminated surfaces and (v) to apply the electrodes in FIA-EC, LC-EC and CE-EC assays with the objective of significantly improving the analytical detection figures of merit.

描述（由申请人提供）：拟议的研究将制造，评估和开发金刚石和氢化碳纤维微电极，用于测量和检测电活性神经递质，相关代谢物和其他生物分析物。这些新型的低氧化物和化学稳定的微电极有可能对体外和体内神经电化学测量产生重大影响，因为它们解决了常见sp2碳纤维微电极经常遇到的几个问题/并发症。这些问题/并发症的存在是因为电活性和可电离的表面碳氧官能团的存在。微电极将研究在直径20-100微米的W和Mo纤维和氢等离子体处理的碳纤维（沥青和pan基，直径10-40微米）上共形沉积硼掺杂微晶和氮掺杂纳米晶金刚石薄膜。与常用的sp2碳电极相比，这些电极材料具有优势，包括较低的伏安和安培背景电流；没有与电活性表面碳氧官能团相关的背景伏安特征；由于缺乏可电离的碳氧官能团，带电溶液分析物的响应灵敏度没有变化；由于非极性、端氢表面，耐结垢；低检出限；提高了响应精度和稳定性。本研究的目标是：(i)制备并全面表征微电极，（ii）证明其在测量神经递质、代谢物和生物分析物方面的功效，（iii）验证低氧化表面解决了当使用氧功能化电极时快速扫描伏安测量中存在的问题/并发症。（iv）详细了解电极在氢端表面的反应动力学和机理；(v)将电极应用于FIA-EC、LC-EC和CE-EC分析，目的是显著提高分析检测指标。