ORGAN CROPRESERVATION USING MOLECULAR ICE CONTROL

描述(由申请人提供)： 捐赠的器官每年被用来延长数千人的生命。不幸的是，目前的需求大大超过了捐赠器官的可获得性，造成了巨大的短缺。由于器官健康在从捐赠者到接受者的运输过程中开始迅速恶化，移植的时间紧迫性使问题变得更加复杂。一种为捐赠器官提供无限期储存的机制将通过取消改进组织分型和延长免疫预适应的时间限制，消除长期冷缺血的有害影响，并提高器官移植的成功。本研究的目的是确定一种在玻璃化过程中使用分子冰控制技术的新的冷冻保存方法是否能够成功地阻止保存过程中的冰形成，并允许在大鼠肾脏模型中长期保存。我们将评估使用天然和合成的分子阻冰化合物配制的玻璃化溶液，这些化合物能够限制冰核和生长。在这项提案中，将进行体外和体内研究，以评估使用分子冰控配方和标准玻璃化溶液玻璃化冷冻后的大鼠肾脏的存活率和功能。预计含有分子冰控制化合物的溶液将比标准玻璃化溶液经历更少的冰形成，并在移植后产生更好的肾功能。

DESCRIPTION (provided by applicant): Donated organs are used to extend life for thousands of people each year. Unfortunately, the availability of donor organs is greatly outweighed by current demand causing enormous shortages. The problem is compounded by the time critical nature of transplantation as the organ health begins to deteriorate rapidly during transport from donor to recipient. A mechanism that provided open-ended storage periods for donated organs would eliminate the detrimental effects of prolonged cold ischemia and improve organ transplant success by lifting time limits for improved tissue-typing and extended immunological preconditioning. The objective of this study is to determine if a new cryopreservation method using molecular ice control technology in a vitrification process can successfully block ice formation during preservation and permit long-term storage in a rat kidney model. We will evaluate vitrification solutions formulated with natural and synthetic molecular ice blocking compounds that are capable of limiting ice nucleation and growth. In this proposal, both in vitro and in vivo studies will be conducted to assess the viability and function of rat kidneys following vitrification with molecular ice control formulations and a standard vitrification solution. It is anticipated that solutions containing molecular ice control compounds will experience less ice formation than a standard vitrification solution and result in better renal function following transplantation.