Using precision diagnostic technologies to tailor tree disease management strategies

利用精准诊断技术制定树木病害管理策略

• 批准号: 2129117
• 金额: --
• 依托单位: University of Lincoln
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2129117
• 关键词: Using; precision; diagnostic; technologies; tailor

For major foliar and trunk pathogens of apple (Venturia inaequalis [scab], Podosphaera leutricha [powdery mildew] and Neonectria ditissima [European apple canker]) there is a wealth of evidence, both for the host relating to major gene resistances, and for the pathogen about population-level variation and structure that is associated with host development. However, this information is currently not applied in the field to inform deployment. Rapid single molecule sequencing of DNA and RNA is now possible using Oxford Nanopore Technology (ONT). The aim of this project is to develop this technology for the rapid in-field monitoring of pathogen populations, in order to inform management strategies. The PhD project fully meets the BBSRC objectives of better countering of diseases of crops and greater resilience of crops to abiotic stresses.NIAB EMR are early access members of the ONT MinION and VolTRAX programmes and have been working for over 14 months on applications for these technologies in the horticultural industry. Currently it is being applied to bacterial and fungal genomes, resulting in complete assembled genomes for many major horticultural pathogens. Bioinformatics pipelines are now well established and significant investment in hardware has been made to ensure that planned future developments in the technology do not impede progress.

对于苹果的主要叶部和树干病原体（黑星菌[黑星病]，Podosphaera leutricha [白粉病]和Neonectria ditissima [欧洲苹果溃疡病]），有大量的证据，无论是与主要基因抗性相关的宿主，还是与宿主发育相关的种群水平变异和结构的病原体。然而，这一信息目前没有应用于实地部署。DNA和RNA的快速单分子测序现在可以使用牛津纳米孔技术（ONT）。该项目的目的是开发这种技术来快速现场监测病原体种群，以便为管理策略提供信息。该博士项目完全符合BBSRC的目标，即更好地对抗作物疾病，提高作物对非生物胁迫的适应能力。NIAB EMR是ONT MinION和VolTRAX计划的抢先体验成员，并已在园艺行业应用这些技术超过14个月。目前，它正被应用于细菌和真菌的基因组，导致许多主要的园艺病原体的完整组装的基因组。生物信息学管道现已建立，并在硬件方面进行了大量投资，以确保计划中的未来技术发展不会阻碍进展。