3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
ATPase HRS-2 的 3D 电子显微镜
基本信息
- 批准号:6622949
- 负责人:
- 金额:$ 28.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-04-01 至 2006-03-31
- 项目状态:已结题
- 来源:
- 关键词:adenosine triphosphate adenosinetriphosphatase bioimaging /biomedical imaging chemical association conformation cryoelectron microscopy electron microscopy enzyme activity enzyme inhibitors monomer mutant phosphoproteins point mutation protein binding protein protein interaction protein structure function structural biology syntaxin
项目摘要
DESCRIPTION (provided by applicant): Hrs-2 is an ATPase that has been
implicated in a variety of cellular processes by virtue of its functional
protein interactions. We have determined that the form of hrs-2 with the
highest specific ATPase activity is an oligomer of six subunits. However, the
structure of this complex is unknown. Consequently, the extensive functional
studies lack a structural basis for their interpretation. Our goal is to use
three-dimensional electron microscopy to determine the structure of hrs-2 and
its functional complexes. We show in the preliminary results that hrs-2 can be
isolated in sufficient quantities to visualize it in the electron microscope
and that the data collected is of sufficient quality to perform a
three-dimensional reconstruction of this protein.
Association of hrs-2 with interacting proteins as well as its intrinsic ATPase
activity likely plays a role in its function. Within the framework of this
proposal, we will investigate the role of the ATPase activity and interacting
proteins in the structure of the hrs-2 oligomer. To achieve this goal, we will
employ three-dimensional electron microscopy and further refine the results
using computational structure prediction methods. This structural information
will provide insight into the role of interacting proteins and enzyme activity
on the conformation of the hrs-2 oligomer and ultimately on its function. The
Specific Aims are: (1) Three-dimensional electron microscopy of the native
structure of hrs-2. (2) High-resolution structure of the hrs-2 by cryo-electron
microscopy and structure prediction methods. (3) Determination of the role of
hrs-2-interacting proteins on the structure of hrs-2. (4) Determination of the
effect of ATP binding on the structure of hrs-2. (5) Determination of the
structural basis for the mechanism by which hrs-2 inhibits SNARE complex
formation.
描述(由申请人提供):Hrs-2是一种atp酶
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
PAWEL A. PENCZEK其他文献
PAWEL A. PENCZEK的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('PAWEL A. PENCZEK', 18)}}的其他基金
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
ATPase HRS-2 的 3D 电子显微镜
- 批准号:
6729851 - 财政年份:2002
- 资助金额:
$ 28.25万 - 项目类别:
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
ATPase HRS-2 的 3D 电子显微镜
- 批准号:
6881678 - 财政年份:2002
- 资助金额:
$ 28.25万 - 项目类别:
相似海外基金
MOLECULAR CHARACTERIZATION OF THE SODIUM POTASSIUM TRANSPORT ADENOSINETRIPHOSPHATASE
钠钾转运腺苷三磷酸酶的分子表征
- 批准号:
7461764 - 财政年份:1974
- 资助金额:
$ 28.25万 - 项目类别:
MOLECULAR CHARTERIZATION OF THE SODIUM-POTASSIUM TRANSPORT ADENOSINETRIPHOSPHATASE
钠钾转运腺苷三磷酸酶的分子表征
- 批准号:
7352845 - 财政年份:1973
- 资助金额:
$ 28.25万 - 项目类别:
Molecular Characterization of the Sodium-Potassiumtransport Adenosinetriphosphatase
钠钾转运三磷酸腺苷酶的分子表征
- 批准号:
7301506 - 财政年份:1973
- 资助金额:
$ 28.25万 - 项目类别:
Continuing Grant
MOLECULAR CHARACTERIZATION OF THE SODIUM-POTASSIUM TRANSPORT ADENOSINETRIPHOSPHATASE
钠钾转运腺苷三磷酸酶的分子表征
- 批准号:
7243716 - 财政年份:1972
- 资助金额:
$ 28.25万 - 项目类别:
MOLECULAR CHARACTERIZATION OF THE SODIUM-POTASSIUM TRANSPORT ADENOSINETRIPHOSPHATASE
钠钾转运腺苷三磷酸酶的分子表征
- 批准号:
7138222 - 财政年份:1971
- 资助金额:
$ 28.25万 - 项目类别:
Molecular Characterization of the Sodium-Potassium Transport Adenosinetriphosphatase
钠钾转运三磷酸腺苷酶的分子表征
- 批准号:
6928993 - 财政年份:1969
- 资助金额:
$ 28.25万 - 项目类别:
Adenosinetriphosphatase Genesis in Bone Marrow Cells
骨髓细胞中腺苷三磷酸酶的发生
- 批准号:
64B2295 - 财政年份:1964
- 资助金额:
$ 28.25万 - 项目类别:
Adenosinetriphosphatase and sugar Transport Mechanism
三磷酸腺苷酶和糖转运机制
- 批准号:
6216854 - 财政年份:1962
- 资助金额:
$ 28.25万 - 项目类别:
Bone Marrow Cells and Relation to Adenosinetriphosphatase Activity
骨髓细胞及其与三磷酸腺苷酶活性的关系
- 批准号:
6216803 - 财政年份:1962
- 资助金额:
$ 28.25万 - 项目类别:














{{item.name}}会员




