3D ELECTRON MICROSCOPY OF THE ATPase HRS-2

描述（由申请人提供）：Hrs-2是一种ATP酶， 由于其功能性，参与各种细胞过程 蛋白质相互作用我们已经确定，hrs-2的形式与 ATP酶比活性最高的是六个亚基的寡聚体。但 这个复杂的结构是未知的。因此，广泛的功能 研究缺乏解释的结构基础。我们的目标是利用 三维电子显微镜以确定HRs-2的结构， 其功能复合物。我们在初步结果中表明，hrs-2可以 分离出足够的量在电子显微镜下观察 所收集的数据质量足以执行 这个蛋白质的三维重建。 hrs-2与相互作用蛋白及其内源性ATP酶的结合 活动可能在其功能中发挥作用。在此框架内， 建议，我们将研究ATP酶活性和相互作用的作用， HRs-2寡聚体结构中的蛋白质。为了实现这一目标，我们将 使用三维电子显微镜， 使用计算结构预测方法。这些结构信息 将提供深入了解相互作用的蛋白质和酶活性的作用， 取决于HRs-2寡聚体的构象并最终取决于其功能。的 具体目的是：（1）研究天然植物的三维电子显微镜 HRs-2的结构。(2)hrs-2的冷电子高分辨结构 显微镜和结构预测方法。(3)确定联合国的作用 hrs-2-相互作用蛋白对hrs-2结构的影响。(4)测定 ATP结合对hrs-2结构的影响。(5)测定 hrs-2抑制SNARE复合物机制的结构基础 阵

DESCRIPTION (provided by applicant): Hrs-2 is an ATPase that has been implicated in a variety of cellular processes by virtue of its functional protein interactions. We have determined that the form of hrs-2 with the highest specific ATPase activity is an oligomer of six subunits. However, the structure of this complex is unknown. Consequently, the extensive functional studies lack a structural basis for their interpretation. Our goal is to use three-dimensional electron microscopy to determine the structure of hrs-2 and its functional complexes. We show in the preliminary results that hrs-2 can be isolated in sufficient quantities to visualize it in the electron microscope and that the data collected is of sufficient quality to perform a three-dimensional reconstruction of this protein. Association of hrs-2 with interacting proteins as well as its intrinsic ATPase activity likely plays a role in its function. Within the framework of this proposal, we will investigate the role of the ATPase activity and interacting proteins in the structure of the hrs-2 oligomer. To achieve this goal, we will employ three-dimensional electron microscopy and further refine the results using computational structure prediction methods. This structural information will provide insight into the role of interacting proteins and enzyme activity on the conformation of the hrs-2 oligomer and ultimately on its function. The Specific Aims are: (1) Three-dimensional electron microscopy of the native structure of hrs-2. (2) High-resolution structure of the hrs-2 by cryo-electron microscopy and structure prediction methods. (3) Determination of the role of hrs-2-interacting proteins on the structure of hrs-2. (4) Determination of the effect of ATP binding on the structure of hrs-2. (5) Determination of the structural basis for the mechanism by which hrs-2 inhibits SNARE complex formation.