3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
批准号:
6729851
负责人:
PAWEL A. PENCZEK
金额:
$28.22万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-04-01 至 2006-03-31
关键词:
adenosine triphosphateadenosinetriphosphatasebioimaging /biomedical imagingchemical associationconformationcryoelectron microscopyelectron microscopyenzyme activityenzyme inhibitorsmonomermutantphosphoproteinspoint mutationprotein bindingprotein protein interactionprotein structure functionstructural biologysyntaxin
中文摘要
描述(由申请人提供):Hrs-2是一种ATP酶,
由于其功能性,参与各种细胞过程
蛋白质相互作用我们已经确定,hrs-2的形式与
ATP酶比活性最高的是六个亚基的寡聚体。但
这个复杂的结构是未知的。因此,广泛的功能
研究缺乏解释的结构基础。我们的目标是利用
三维电子显微镜以确定HRs-2的结构,
其功能复合物。我们在初步结果中表明,hrs-2可以
分离出足够的量在电子显微镜下观察
所收集的数据质量足以执行
这个蛋白质的三维重建。
hrs-2与相互作用蛋白及其内源性ATP酶的结合
活动可能在其功能中发挥作用。在此框架内,
建议,我们将研究ATP酶活性和相互作用的作用,
HRs-2寡聚体结构中的蛋白质。为了实现这一目标,我们将
使用三维电子显微镜,
使用计算结构预测方法。这些结构信息
将提供深入了解相互作用的蛋白质和酶活性的作用,
取决于HRs-2寡聚体的构象并最终取决于其功能。的
具体目的是:(1)研究天然植物的三维电子显微镜
HRs-2的结构。(2)hrs-2的冷电子高分辨结构
显微镜和结构预测方法。(3)确定联合国的作用
hrs-2-相互作用蛋白对hrs-2结构的影响。(4)测定
ATP结合对hrs-2结构的影响。(5)测定
hrs-2抑制SNARE复合物机制的结构基础
阵
英文摘要
DESCRIPTION (provided by applicant): Hrs-2 is an ATPase that has been
implicated in a variety of cellular processes by virtue of its functional
protein interactions. We have determined that the form of hrs-2 with the
highest specific ATPase activity is an oligomer of six subunits. However, the
structure of this complex is unknown. Consequently, the extensive functional
studies lack a structural basis for their interpretation. Our goal is to use
three-dimensional electron microscopy to determine the structure of hrs-2 and
its functional complexes. We show in the preliminary results that hrs-2 can be
isolated in sufficient quantities to visualize it in the electron microscope
and that the data collected is of sufficient quality to perform a
three-dimensional reconstruction of this protein.
Association of hrs-2 with interacting proteins as well as its intrinsic ATPase
activity likely plays a role in its function. Within the framework of this
proposal, we will investigate the role of the ATPase activity and interacting
proteins in the structure of the hrs-2 oligomer. To achieve this goal, we will
employ three-dimensional electron microscopy and further refine the results
using computational structure prediction methods. This structural information
will provide insight into the role of interacting proteins and enzyme activity
on the conformation of the hrs-2 oligomer and ultimately on its function. The
Specific Aims are: (1) Three-dimensional electron microscopy of the native
structure of hrs-2. (2) High-resolution structure of the hrs-2 by cryo-electron
microscopy and structure prediction methods. (3) Determination of the role of
hrs-2-interacting proteins on the structure of hrs-2. (4) Determination of the
effect of ATP binding on the structure of hrs-2. (5) Determination of the
structural basis for the mechanism by which hrs-2 inhibits SNARE complex
formation.
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专著(0)
科研奖励(0)
会议论文
TRD2: Phasing and refinement
-
批准号:10155528
-
项目类别:
-
资助金额:$24.83万
-
财政年份:2020
-
负责人:PAWEL A. PENCZEK
-
依托单位:
TRD2: Phasing and refinement
-
批准号:10460923
-
项目类别:
-
资助金额:$24.47万
-
财政年份:2020
-
负责人:PAWEL A. PENCZEK
-
依托单位:
TRD2: Phasing and refinement
-
批准号:10641818
-
项目类别:
-
资助金额:$24.47万
-
财政年份:2020
-
负责人:PAWEL A. PENCZEK
-
依托单位:
UNIVERSITY OF TEXAS SCHOOL OF MEDICINE
-
批准号:8151952
-
项目类别:
-
资助金额:$15.46万
-
财政年份:2010
-
负责人:PAWEL A. PENCZEK
-
依托单位:
3D ELECTRON MICROSCOPY OF HRS
-
批准号:7183092
-
项目类别:
-
资助金额:$1.31万
-
财政年份:2005
-
负责人:PAWEL A. PENCZEK
-
依托单位:
POSTTRANSLATIONAL PROTEIN CONDUCTING CHANNEL
-
批准号:7181093
-
项目类别:
-
资助金额:$1.85万
-
财政年份:2004
-
负责人:PAWEL A. PENCZEK
-
依托单位:
GLOBAL OPTIMIZATION OF REFINEMENT PARAMETERS
-
批准号:6757130
-
项目类别:
-
资助金额:$19.14万
-
财政年份:2003
-
负责人:PAWEL A. PENCZEK
-
依托单位:
POSTTRANSLATIONAL PROTEIN CONDUCTING CHANNEL
-
批准号:6980404
-
项目类别:
-
资助金额:$1.08万
-
财政年份:2003
-
负责人:PAWEL A. PENCZEK
-
依托单位:
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
-
批准号:6622949
-
项目类别:
-
资助金额:$28.25万
-
财政年份:2002
-
负责人:PAWEL A. PENCZEK
-
依托单位:
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
-
批准号:6459418
-
项目类别:
-
资助金额:$28.35万
-
财政年份:2002
-
负责人:PAWEL A. PENCZEK
-
依托单位:
3D ELECTRON MICROSCOPY OF THE ATPase HRS-2
-
批准号:6881678
-
项目类别:
-
资助金额:$28.22万
-
财政年份:2002
-
负责人:PAWEL A. PENCZEK
-
依托单位:
HIGH RESOLUTION IN SINGLE PARTICLE RECONSTRUCTION
-
批准号:6690997
-
项目类别:
-
资助金额:$16.99万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
High Resolution in Single Particle Reconstruction
-
批准号:6865030
-
项目类别:
-
资助金额:$25.12万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
High Resolution in Single Particle Reconstruction
-
批准号:8324544
-
项目类别:
-
资助金额:$29.86万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
High Resolution in Single Particle Reconstruction
-
批准号:7931996
-
项目类别:
-
资助金额:$30.16万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
High Resolution in Single Particle Reconstruction
-
批准号:7163510
-
项目类别:
-
资助金额:$24.72万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
HIGH RESOLUTION IN SINGLE PARTICLE RECONSTRUCTION
-
批准号:6490233
-
项目类别:
-
资助金额:$16.12万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
HIGH RESOLUTION IN SINGLE PARTICLE RECONSTRUCTION
-
批准号:6627280
-
项目类别:
-
资助金额:$16.54万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
HIGH RESOLUTION IN SINGLE PARTICLE RECONSTRUCTION
-
批准号:6041404
-
项目类别:
-
资助金额:$2.67万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
HIGH RESOLUTION IN SINGLE PARTICLE RECONSTRUCTION
-
批准号:6334478
-
项目类别:
-
资助金额:$15.26万
-
财政年份:2000
-
负责人:PAWEL A. PENCZEK
-
依托单位:
海外基金