Revealing the inner workings of the bacterial growth and division by super-resolution microscopy

通过超分辨率显微镜揭示细菌生长和分裂的内部运作

• 批准号: 2143139
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2143139
• 关键词: Revealing; inner; workings; bacterial; growth

Given the ever-increasing prevalence of multi-drug resistant pathogens, the need to identify novel antimicrobials and antimicrobial targets is higher than ever. The highly conserved and essential nature of many bacterial cell growth and division proteins, coupled with a lack of eukaryotic homologues, make these proteins an ideal target for novel antimicrobials. In this project, we aim to use super-resolution microscopy to gain insight into the organisation and dynamics of key proteins within the bacterial division and elongation complexes. This world class basic bioscience study exploits multiple new ways of working via international partnerships & collaboration: bioimaging/ super-resolution microscopy, nanotechnology, cutting edge biological chemistry, single cell bacterial physiology and quantitative analysis of massive and complex image data. The study will directly address the "World class underpinning Biosciences" BBSRC strategic priority, by continuing a cutting-edge international collaboration. It also addresses Bioscience for Health Strategic Priority 3 since an improved understanding of the mechanisms of bacterial cell division and growth may enable identification of new targets for antimicrobials.

鉴于多重耐药病原体的流行率不断增加，确定新型抗菌剂和抗菌靶标的需求比以往任何时候都高。许多细菌细胞生长和分裂蛋白的高度保守和必需性质，加上缺乏真核同源物，使这些蛋白质成为新型抗菌剂的理想靶标。在这个项目中，我们的目标是使用超分辨率显微镜来深入了解细菌分裂和延伸复合物中关键蛋白质的组织和动力学。这项世界级的基础生物科学研究通过国际伙伴关系和合作利用了多种新的工作方式：生物成像/超分辨率显微镜，纳米技术，尖端生物化学，单细胞细菌生理学和大量复杂图像数据的定量分析。该研究将通过继续进行尖端的国际合作，直接解决“世界级支撑生物科学”的BBSRC战略优先事项。它还涉及生物科学促进健康战略优先事项3，因为更好地了解细菌细胞分裂和生长的机制可能有助于确定抗菌剂的新目标。