ORTHOPOXVIRUS GENOMIC AND BIOINFORMATICS RESOURCE CENTER

针对天花病毒等正痘病毒可能被用作恐怖分子的武器，我们建议建立一个正痘病毒遗传和生物信息学资源中心，收集、编目、注释和分析与该病毒属有关的遗传信息。这项工作将在逐个基因和全基因组的基础上扩展病毒复制和病毒-宿主相互作用的现有知识。然后，这些信息将提供给科学界，以支持目前开发环境探测器、诊断方法、抗病毒化合物、新疫苗和动物模型的研究工作。为了支持这些目标，我们建议：开发一个关系数据库，支持本提案的数据存储、注释、分析和信息交换目标。2. 发展一个世界范围的网站，使这项工作所汇编和产生的所有资料和分析结果能够向科学界公布，使研究人员能够根据个别研究人员的需要迅速取得具体资料。3. 收集现有正痘病毒物种的基因和基因组序列，并将其输入数据库，供后续注释和分析；提供对现有和新分离的正痘病毒基因组部分进行测序的能力，以便与现有毒株和物种进行比较；并为收集到的任何新分离的病毒提供活病毒样本和病毒克隆库。4. 提供计算机自动和人为的注释，以确认和更新现有和新获得的序列记录中的现有生物信息；并对基因组和基因序列进行各种分析，以提供有关其结构，功能和进化的额外信息。

In response to the potential use of orthopoxviruses such as variola virus as a terrorist weapon, we propose to establish an Orthopoxvirus Genetic and Bioinformatics Resource Center that will collect, catalog, annotate, and analyze genetic information related to this virus genus. This work will expand on available knowledge of virus replication and virus-host interaction on a gene-by-gene and whole genome basis. This information will then be made available to the scientific community to support current research efforts to develop environmental detectors, diagnostics, antiviral compounds, new vaccines, and animal models. In support of these goals, we propose: 1. To develop a relational database that will support the data storage, annotation, analysis, and information exchange goals of this proposal. 2. To develop a world-wide web site that will allow all information and analytical results compiled and generated by this work to be publicized to the scientific community, providing rapid access to specific information as required by an individual researcher. 3. To collect existing gene and genomic sequences for orthopoxvirus species and import them into the database for subsequent annotation and analysis; to provide the capability to sequence portions of the genomes from existing and newly isolated orthopoxviruses for comparison with existing strains and species; and to provide a repository for live virus samples and virus clones for any newly isolated viruses that have been collected. 4. To provide computer-automated and human-directed annotation to confirm and update existing biological information in the sequence records for existing as well as newly obtained sequences; and to perform a variety of analyses on genome and gene sequences to provide additional information on their structure, function, and evolution.