To explore synthetic biology approaches for in vivo data storage

探索体内数据存储的合成生物学方法

• 批准号: 2202898
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2202898
• 关键词: explore; synthetic; biology; approaches; vivo

Approach to researchIn order to reach my research, aim I am conducting the following:* Data gathering and analysis of how to modify the current design so that it will work with the machinery within the cell whilst remaining protected.* Experiments to test its performance in prokaryotes (E. coli is the prokaryote we will start with)I am using many synthetic biology processes and nanotechnology concepts to achieve this. Synthetic biology is the redesigning or creation of new biological systems and nanotechnology is the creation of devices on a nanoscaleBackgroundTraditional data storage media comprises of optical, magnetic and semiconductor memory. The major problem with it is the incoming "Data Apocalypse". The "Data Apocalypse" is a so-called event that society runs out of data storage. This event could happen as data creation is currently outstripping data storage media. Data creation is currently trending at a rate of 2.5 quintillion bytes per day which equates 10^18 Bytes. This is combined with the environmental cost of traditional data storage, it has been forecasted by many analysts that data centres are responsible for 2% of the global greenhouse gas emissions, making them equivalent to the aviation industry. This is only predicted to get worse especially with the ever-increasing dependency on the internet and other technologies in modern day life. Thus, a new technology is necessary. In early 2013, the idea of using DNA (Deoxyribonucleic Acid) to store information started to gather funding as its more information dense and environmentally friendlier. The method currently used to store information on DNA uses an encoding algorithm and decoding algorithm to "write" bits of information onto nucleotides. My project seeks to improve, optimise and extend the state of the art by looking at computer aided design for synthetic biology and the marshalling of DNA/RNA nanotechnologies.Future applicationsThroughout this I will be developing several novel processes such as how to engineer DNA and RNA structures so that they are compatible in prokaryotes which will not just be relevant to in vivo data storage but can have applications in the medical therapeutic industry to name but one.

研究方法为了实现我的研究目标，我正在进行以下工作：* 收集和分析如何修改当前设计，使其能够与细胞内的机械一起工作，同时保持受保护。* 测试其在原核生物中的性能的实验（大肠杆菌是我们将开始的原核生物）我正在使用许多合成生物学过程和纳米技术概念来实现这一目标。合成生物学是重新设计或创建新的生物系统，纳米技术是在纳米尺度上创建设备背景传统的数据存储介质包括光、磁和半导体存储器。它的主要问题是即将到来的“数据启示录”。 “数据启示录”是所谓的社会数据存储耗尽的事件。由于数据创建目前超过了数据存储介质，因此可能会发生此事件。目前数据创建趋势为每天 2.5 万亿字节，相当于 10^18 字节。再加上传统数据存储的环境成本，许多分析师预测，数据中心排放的温室气体占全球温室气体排放量的2%，相当于航空业。预计这种情况只会变得更糟，尤其是随着现代生活对互联网和其他技术的依赖不断增加。因此，需要一种新技术。 2013 年初，利用 DNA（脱氧核糖核酸）存储信息的想法开始获得资金支持，因为它的信息密度更大，也更环保。目前用于在DNA上存储信息的方法使用编码算法和解码算法将信息位“写入”到核苷酸上。我的项目旨在通过研究合成生物学的计算机辅助设计和 DNA/RNA 纳米技术的编组来改进、优化和扩展现有技术。未来的应用在此过程中，我将开发几种新颖的过程，例如如何设计 DNA 和 RNA 结构，以便它们在原核生物中兼容，这不仅与体内数据存储相关，而且可以在医学治疗中应用 仅举一个行业。