GENETIC AND MOLECULAR STUDY OF MEIOTIC TRANSVECTION

减数分裂传递的遗传和分子研究

• 批准号: 6627292
• 负责人: Rodolfo Aramayo
• 金额: $ 22.47万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6627292
• 关键词: DNA binding protein; Neurospora; alleles; biological models; cytogenetics; diploidy; eukaryote; gene expression; gene mutation; genetic regulation; genetic regulatory element; genetic transcription; meiosis; molecular genetics; northern blottings; posttranscriptional RNA processing; sex differentiation; southern blotting; suppressor mutations

This work is aimed at understanding meiotic transvection, a mechanism of gene regulation that controls gene expression in chromosome pairing-dependent manner. Neurospora is a particularly attractive system for this study because it is the only microorganism in which transvection has been observed and the only organism of any kind in which transvection has been observed in meiosis. The long term objective of this research is to understand how meiotic transvection works, to identify the components of its molecular machinery, and to investigate if this chromosome pairing-dependent mechanism of gene regulation exists in other eukaryotes. Our studies on the molecular basis of chromosome pairing- dependent gene control in Neurospora are expected to aid our understanding of similar mechanisms in humans, where they may be responsible for causing disease. To understand meiotic transvection two interrelated projects will be developed. First, the minimal cis-acting signals responsible for the pairing-dependent trans-regulation of the Ascospore maturation-1 (Asm-1+) gene will be determined, and studied, by classical and molecular genetics. These minimal cis-acting signals will then be used to identify Neurospora proteins that bind to this DNA fragment. Second the transcriptional and post- transcriptional regulation of paired and unpaired copies of Asm- 1+ during sexual development will be studied, and the molecular basis of the dominant behavior of unpaired copies of the gene will be determined. Our understanding of the molecular mechanisms, and of the cellular components involved in meiotic transvection will contribute to the identification, study and understanding of mechanisms of gene control based on diploidy, the dominant phase of most eukaryotes.

这项工作的目的是了解减数分裂的transvection， 控制基因表达的基因调控机制， 染色体配对依赖的方式。 脉孢菌是一种 这项研究特别有吸引力的系统，因为它是 仅观察到转染的微生物， 是唯一一种 在减数分裂中观察到的。 这项研究的长期目标是了解如何 减数分裂转染工程，以确定其组成部分， 分子机制，并研究这条染色体 配对依赖的基因调控机制存在于其他 真核生物 我们对染色体分子基础的研究 脉孢菌中依赖配对的基因控制有望有助于 我们对人类类似机制的理解， 对引起疾病负责。 为了理解减数分裂的转导，两个相互关联的项目将 发展。 第一，最小的顺式作用信号负责 对于子囊孢子的配对依赖性反式调节 成熟-1（Asm-1+）基因将由以下人员确定和研究 经典和分子遗传学。 这些最小的顺式作用 信号将被用来识别脉孢菌蛋白， 与该DNA片段结合。 第二，转录和后 Asm的配对和未配对拷贝的转录调节- 1+在性发育过程中将被研究， 基因的不成对拷贝的显性行为的基础 将被确定。 我们对分子机制和细胞机制的理解 参与减数分裂转染的组分将有助于 基因控制机制的鉴定、研究和理解 基于二倍体，大多数真核生物的优势阶段。