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Genetic and Molecular Study of Meiotic Trans-sensing and Meiotic Silencing

减数分裂转感和减数分裂沉默的遗传和分子研究

基本信息

批准号：
7545447
负责人：
Rodolfo Aramayo
金额：
$ 27.6万
依托单位：
TEXAS A&M UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-01-01 至 2011-12-31
项目状态：
已结题

项目摘要

Homology-sensing mechanisms are critical to meiosis. How homology-sensing is used by chromosomes to find their pairing partners is still a mystery. Yet, genome integrity and the faithful transmission of the genetic information contained in chromosomes depend on these mechanisms. Chromosome pairing is an important and conserved mechanical event. Our working hypothesis is that this mechanical pairing is, in addition, an active "sensing" mechanism used by all meiotic chromosomesto examine their homologues from stem to stern in each meiosis. In this process, chromosomes use the sophisticated meiotic trans-sensing machinery to evaluate their pairing partners. Failure of "sensing" or "pairing" of a discretevDNA region because of the presence of a heterologous region, or the presence of a deletion on the opposite chromosome (i.e., the lack of a homologous region), triggers meiotic silencing-a novel meiotic RNA silencing mechanism. Once activated, the meiotic silencing machinery produces a diffusible silencing signal specific to the unpaired DNA. This signal then, silences the expression of all genes homologousto the ones contained in the unpaired region, whether they are paired or unpaired. The objective of this research is to identify all the players that participate in, and to dissect the molecular mechanisms used by meiotic trans-sensing and meiotic silencing in Neurospora. During this funding period we will develop a series of interrelated projects aimed at defining how homologs "sense" each other and at characterizing mutants affected in these processes. Understanding chromosome sensing is important, for it will lead us to a better understanding of the surveillance mechanisms used by cells to detect "foreign" sequences and how these surveillance mechanisms work to inactivate them, knowledge that has great potential for the therapeutic manipulation of gene expression in all organisms. Understanding these mechanisms can aid our understanding of similar mechanisms in humans, where they participate in both development and disease.

同源感应机制对减数分裂至关重要。同源感应是如何被染色体用来找到它们的配对伴侣仍然是个谜。然而，基因组的完整性和基因的忠实传递包含在染色体中的信息依赖于这些机制。染色体配对是一种重要的和保守的机械事件。我们的工作假设是，这种机械配对是，此外，所有减数分裂染色体都使用一种主动的“感应”机制来检测它们的同源物，在每一次减数分裂中都是严峻的。在这个过程中，染色体使用复杂的减数分裂跨传感机制来评估他们的配对伙伴由于不连续的DNA区域的“感应”或“配对”失败，异源区域的存在，或相对染色体上缺失的存在（即，缺乏的同源区域），触发减数分裂沉默-一种新的减数分裂RNA沉默机制。一旦当减数分裂沉默机制被激活时，会产生一种特异于未配对染色体的可扩散的沉默信号。 DNA.然后，这个信号沉默了所有基因的表达，这些基因与包含在基因组中的基因同源。未配对的区域，无论它们是配对的还是未配对的。这项研究的目的是确定所有的参与者，并剖析减数分裂trans-sensing所使用的分子机制，脉孢菌减数分裂沉默。在此期间，我们将开展一系列相关项目旨在定义同源物如何相互“感知”，并描述这些同源物中受影响的突变体。流程.了解染色体传感是很重要的，因为它将引导我们更好地理解细胞用来检测“外来”序列的监视机制以及这些监视机制如何机制的工作，以消除他们，知识，有很大的潜力，为治疗操纵所有生物体的基因表达。理解这些机制可以帮助我们理解类似的在人类中，它们参与了发育和疾病。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Construction of strains for rapid homokaryon purification after integration of constructs at the histidine-3 ( his-3) locus of Neurospora crassa.

在粗糙脉孢菌的组氨酸 3 (his-3) 基因座上整合构建体后，构建用于快速同核纯化的菌株。

DOI：
10.1007/s00294-002-0366-z
发表时间：
2003
期刊：
Current genetics
影响因子：
2.5
作者：
Lee,DongW;Haag,JeremyR;Aramayo,Rodolfo
通讯作者：
Aramayo,Rodolfo

Draft de novo transcriptome assembly and proteome characterization of the electric lobe of Tetronarce californica: a molecular tool for the study of cholinergic neurotransmission in the electric organ.

加州Tetronarce californica电叶的从头转录组组装和蛋白质组表征草案：用于研究电器官中胆碱能神经传递的分子工具。

DOI：
10.1186/s12864-017-3890-4
发表时间：
2017
期刊：
BMC genomics
影响因子：
4.4
作者：
Stavrianakou,Maria;Perez,Ricardo;Wu,Cheng;Sachs,MatthewS;Aramayo,Rodolfo;Harlow,Mark
通讯作者：
Harlow,Mark

The G1 cyclin Cln3p controls vacuolar biogenesis in Saccharomyces cerevisiae.

G1 细胞周期蛋白 Cln3p 控制酿酒酵母中的液泡生物发生。