Effects of organochlorines on male infertility

描述(由申请人提供)：目前有10%至17%的美国夫妇因不孕不育而寻求医疗帮助；这些问题中有一半是男性原因，通常病因不明。野生动物、实验动物和人类的流行病学研究表明，环境污染物，特别是多氯联苯(PCbs)和有机氯农药(OC)等有机氯化合物(OCS)对男性生殖有不利影响。为了研究人类生殖健康，特别是精液质量与生殖激素水平(FSH、LH、睾酮和抑制素B，被认为是最有效的精子发生内分泌标记物)、OC环境污染物以及与污染物和性类固醇代谢相关的基因多态性(P450)之间的关系，我们提议进行一项病例对照研究，从密歇根州底特律的一例不育患者中招募受试者。病例将是低精子密度(小于2x10x6/ml)的男性，对照组(2组)将是精子密度大于2但小于20x10x6/ml的男性，以及精子密度正常(大于20x10x6/ml)的男性。将从一份自填式问卷中获得关于五大湖运动捕捞鱼类消费以及其他风险因素的信息。二次暴露(五大湖鱼类中存在的污染物)，包括多氯联苯和有机碳杀虫剂，将从血样中测量。将在颊(颊)细胞DNA中检测P450基因的多态。这项针对五大湖地区已知有机碳污染和大量垂钓者的低精子密度男性病例对照研究将提供数据，帮助确定五大湖运动捕捞的鱼的消费是否对男性生殖健康的衡量标准产生不利影响。

DESCRIPTION (provided by applicant): Ten to 17% of American couples currently seek medical help for infertility; half of these problems have a male cause usually of unknown etiology. Wildlife, experimental animal and human epidemiological studies indicate that environmental contaminants, especially organochlorine compounds (OCs) such as polychlorinated biphenyls (PCBs) and OC pesticides have adverse effects on male reproduction. To investigate the relationship between measure of human male reproductive health, specifically semen quality and reproductive hormone levels (FSH, LH, testosterone and inhibin B, considered to be the best available endocrine marker of spermatogenesis), OC environmental contaminants and polymorphisms in genes involved in contaminant and sex steroid metabolism (P450), we propose to conduct a case-control study recruiting subjects based on sperm density from an infertility in Detroit, Michigan. Cases will be men with low sperm densities (less than 2 x10x6/ml), and controls (2 groups) will be men with sperm densities greater than 2 but less than 20x10x6/ml, and men with normal sperm densities (greater than 20x10x6/ml). Information on Great Lakes sport-caught fish consumption, as well as on other risk factors will be obtained from a self-administered questionnaire. Secondary exposures (contaminants present in Great Lakes fish), including PCBs and OC pesticides will be measured from a blood sample. P450 polymorphisms will be measured in buccal (cheek) cell DNA. This adequately powered case-control study of men with low sperm densities from an area of the Great Lakes with both known OC contamination and a significant percentage of anglers will provide data that will help determine if consumption of Great Lakes sports-caught fish has or does not have adverse effects on measures of male reproductive health.