Effects of organochlorines on male infertility

描述（由申请人提供）：目前有10%至17%的美国夫妇因不孕症寻求医疗帮助;这些问题中有一半是由男性引起的，通常病因不明。野生动物、实验动物和人类的流行病学研究表明，环境污染物，特别是有机氯化合物（OC），如多氯联苯（PCBs）和OC农药，对雄性生殖有不利影响。探讨男性生殖健康指标，特别是精液质量与生殖激素水平的关系（FSH、LH、睾酮和睾酮素B，被认为是精子发生的最佳内分泌标志物），OC环境污染物和污染物和性类固醇代谢相关基因的多态性（P450），我们建议进行一项病例对照研究，从密歇根州底特律市的一名不育患者中招募基于精子密度的受试者。病例为精子密度低（小于2 × 10 x6/ml）的男性，对照组（2组）为精子密度大于2但小于20 × 10 x6/ml的男性，以及精子密度正常（大于20 × 10 x6/ml）的男性。关于五大湖运动捕获鱼类消费以及其他风险因素的信息将通过自填问卷获得。二次接触（存在于五大湖鱼类中的污染物），包括多氯联苯和有机氯农药，将从血液样本中进行测量。将在颊（颊）细胞DNA中测量P450多态性。这一充分有力的病例对照研究的男性精子密度低，从一个地区的五大湖与已知的OC污染和一个显着的百分比的垂钓者将提供数据，将有助于确定消费的五大湖体育捕捞的鱼有或没有对男性生殖健康的措施产生不利影响。

DESCRIPTION (provided by applicant): Ten to 17% of American couples currently seek medical help for infertility; half of these problems have a male cause usually of unknown etiology. Wildlife, experimental animal and human epidemiological studies indicate that environmental contaminants, especially organochlorine compounds (OCs) such as polychlorinated biphenyls (PCBs) and OC pesticides have adverse effects on male reproduction. To investigate the relationship between measure of human male reproductive health, specifically semen quality and reproductive hormone levels (FSH, LH, testosterone and inhibin B, considered to be the best available endocrine marker of spermatogenesis), OC environmental contaminants and polymorphisms in genes involved in contaminant and sex steroid metabolism (P450), we propose to conduct a case-control study recruiting subjects based on sperm density from an infertility in Detroit, Michigan. Cases will be men with low sperm densities (less than 2 x10x6/ml), and controls (2 groups) will be men with sperm densities greater than 2 but less than 20x10x6/ml, and men with normal sperm densities (greater than 20x10x6/ml). Information on Great Lakes sport-caught fish consumption, as well as on other risk factors will be obtained from a self-administered questionnaire. Secondary exposures (contaminants present in Great Lakes fish), including PCBs and OC pesticides will be measured from a blood sample. P450 polymorphisms will be measured in buccal (cheek) cell DNA. This adequately powered case-control study of men with low sperm densities from an area of the Great Lakes with both known OC contamination and a significant percentage of anglers will provide data that will help determine if consumption of Great Lakes sports-caught fish has or does not have adverse effects on measures of male reproductive health.