Effects of organochlorines on male infertility

有机氯对男性不育的影响

• 批准号: 6799600
• 负责人: JULIA Jennifer WIRTH
• 金额: $ 56.12万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-09 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6799600
• 关键词: chlorohydrocarbon insecticide; clinical research; cytochrome P450; diet; environmental contamination; epidemiology; fertility; follicle stimulating hormone; food chain contamination; genetic polymorphism; halobiphenyl /halotriphenyl compound; human middle age (35-64); human subject; inhibin; insecticide biological effect; luteinizing hormone; male; nutrition related tag; patient oriented research; seafood; semen; sperm analysis; spermatogenesis; steroid metabolism; young adult human (21-34)

DESCRIPTION (provided by applicant): Ten to 17% of American couples currently seek medical help for infertility; half of these problems have a male cause usually of unknown etiology. Wildlife, experimental animal and human epidemiological studies indicate that environmental contaminants, especially organochlorine compounds (OCs) such as polychlorinated biphenyls (PCBs) and OC pesticides have adverse effects on male reproduction. To investigate the relationship between measure of human male reproductive health, specifically semen quality and reproductive hormone levels (FSH, LH, testosterone and inhibin B, considered to be the best available endocrine marker of spermatogenesis), OC environmental contaminants and polymorphisms in genes involved in contaminant and sex steroid metabolism (P450), we propose to conduct a case-control study recruiting subjects based on sperm density from an infertility in Detroit, Michigan. Cases will be men with low sperm densities (less than 2 x10x6/ml), and controls (2 groups) will be men with sperm densities greater than 2 but less than 20x10x6/ml, and men with normal sperm densities (greater than 20x10x6/ml). Information on Great Lakes sport-caught fish consumption, as well as on other risk factors will be obtained from a self-administered questionnaire. Secondary exposures (contaminants present in Great Lakes fish), including PCBs and OC pesticides will be measured from a blood sample. P450 polymorphisms will be measured in buccal (cheek) cell DNA. This adequately powered case-control study of men with low sperm densities from an area of the Great Lakes with both known OC contamination and a significant percentage of anglers will provide data that will help determine if consumption of Great Lakes sports-caught fish has or does not have adverse effects on measures of male reproductive health.

描述（由申请人提供）：目前有10 - 17%的美国夫妇因不孕症寻求医疗帮助；这些问题中有一半是男性引起的，通常病因不明。野生动物、实验动物和人类流行病学研究表明，环境污染物，特别是有机氯化合物（OCs），如多氯联苯（PCBs）和有机氯农药，对男性生殖有不利影响。为了研究人类男性生殖健康指标，特别是精液质量与生殖激素水平（FSH、LH、睾酮和抑制素B，被认为是精子发生的最佳内分泌标志物）、OC环境污染物和涉及污染物和性类固醇代谢的基因多态性（P450）之间的关系，我们建议开展一项病例对照研究，招募来自密歇根州底特律的不孕不育患者的精子密度。病例为精子密度低（小于2 × 10x6/ml）的男性，对照组（2组）为精子密度大于2但小于20 × 10x6/ml的男性，以及精子密度正常（大于20 × 10x6/ml）的男性。将从一份自我管理的调查表中获得关于大湖区运动捕捞鱼类消费以及其他危险因素的资料。二次暴露（五大湖鱼类中存在的污染物），包括多氯联苯和有机化合物杀虫剂，将从血液样本中进行测量。P450多态性将在颊（颊）细胞DNA中测量。这项充分有力的病例对照研究对来自五大湖地区的精子密度低的男性进行了研究，该地区既有已知的有机碳污染，也有很大比例的垂钓者。这项研究将提供数据，帮助确定食用五大湖运动捕捞的鱼对男性生殖健康的测量是否有不利影响。