The Evolution of Specialists and Generalists
专家和通才的演变
基本信息
- 批准号:6526199
- 负责人:
- 金额:$ 25.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-08-01 至 2005-07-31
- 项目状态:已结题
- 来源:
- 关键词:Escherichia coli SDS polyacrylamide gel electrophoresis biochemical evolution gene frequency gene mutation genetic polymorphism genetic strain hydrolysis lac operon microarray technology microorganism population study molecular genetics natural selections nuclear magnetic resonance spectroscopy nucleic acid sequence population genetics statistics /biometry systematic biology
项目摘要
DESCRIPTION (provided by applicant): An understanding of microbial evolution is
seen as increasingly important to an understanding of disease and the design of
effective therapies. As such, this proposal aims to investigate the genetic,
biochemical and physiological mechanisms underpinning evolution in microbes. Of
particular interest is an assessment of the degree to which adaptive evolution
is predictable. For if evolution is predictable, more efficacious therapies
might be designed, undesirable evolutionary outcomes (e.g. multi-drug
resistance) avoided, the origin and evolution of epidemics better understood,
and as a consequence better strategies in public health implemented.
An understanding of microbial evolution remains elusive because studies are
either overwhelmed by complexity or reduced to the trivially predictable.
However, the lactose pathway of E. coli represents a unique balance between
complexity and simplicity. It is sufficiently complex that a diversity of
adaptive responses - specialists, generalists, commensals - arise, yet it is
sufficiently simple that each can be analyzed in detail. This balance between
complexity and simplicity enables the predictability of adaptive evolution to
be investigated.
Strong frequency dependent selection maintains variation in lactose operons of
E. coli during competition for limiting mixtures of galactosides. Long-term
chemostat competition experiments reveal that new adaptations may intensify the
frequency dependence or they may diminish it to the point whereby the
polymorphism is lost. Balanced polymorphisms can arise from within a single
clone. The evolution of specialists towards particular galactosides promotes
polymorphism and suggests that trade-offs at the molecular level govern the
evolution of diversity. The evolution of generalists (strains capable of
efficiently metabolizing all galactosides) destabilizes polymorphisms and
demonstrates that trade-offs are not inevitable.
The genetic, physiological and biochemical mechanisms that promote the
appearance, and loss, of these polymorphisms will be determined. Replicate
experiments will determine the predictability of evolutionary outcomes. Evolved
strains will be analyzed to determine the population level interactions that
result in stabilizing or destabilizing the polymorphisms. Biochemical studies
will determine the physiological basis for the evolution of specialists and
generalists, and to determine the molecular causes of trade-oils. Mutational
changes will be sequenced to determine the predictably of evolution at the
molecular level.
描述(由申请人提供):对微生物进化的理解是
项目成果
期刊论文数量(0)
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Antony M. DEAN其他文献
Antony M. DEAN的其他文献
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{{ truncateString('Antony M. DEAN', 18)}}的其他基金
Evolution of a Dehydrogenase in its Adaptive Landscape
脱氢酶在其适应性景观中的进化
- 批准号:
6520158 - 财政年份:2001
- 资助金额:
$ 25.82万 - 项目类别:
Evolution of a Dehydrogenase in its Adaptive Landscape
脱氢酶在其适应性景观中的进化
- 批准号:
6751919 - 财政年份:2001
- 资助金额:
$ 25.82万 - 项目类别:
Evolution of a Dehydrogenase in its Adaptive Landscape
脱氢酶在其适应性景观中的进化
- 批准号:
6317483 - 财政年份:2001
- 资助金额:
$ 25.82万 - 项目类别: