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CORE--CELL ISOLATION AND CULTURE

CORE--CELL ISOLATION AND CULTURE
核心——细胞分离与培养
批准号：
6808274
负责人：
GEORGE COOPER
金额：
$9.95万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
项目状态：
已结题
起止时间：
2003-08-01 至 2008-07-31
来源：
https://reporter.nih.gov/project-details/6808274
关键词：
biomedical facility heart cell heart enlargement intracardiac pressure myocardium pathologic process tissue /cell culture tissue /cell preparation

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中文摘要
翻译
该计划的大部分项目将利用需要分离和表征成人和新生儿心肌细胞或心肌细胞的实验制剂。该核心将从以下动物中分离心肌细胞：1）成年猫，2）成年小鼠，3）新生大鼠和4）新生小鼠。将从正常心脏和压力超负荷肥大的手术模型中分离成人心肌细胞。这些需求最好由核心设施满足，在核心设施中，供应品采购、试剂制备和常规程序可以基于规模经济进行，质量控制由集中的、经验丰富的权威机构进行监测，并通过在集中设施中提供劳动密集型服务节省时间。
英文摘要
The majority of the program projects will utilize experimental preparations that require isolation and characterization of adult and neonatal cardiac muscle cells, or cardiocytes. This core will isolate cardiocytes from: 1) the adult cat, 2) the adult mouse, 3) the neonatal rat, and 4) the neonatal mouse. Adult cardiocytes will be isolated from both normal hearts and surgical models of pressure overload hypertrophy. These demands will best be met by a Core Facility where supply purchases, reagent preparation, and routine procedures can be performed with an economy based on scale, with quality control monitored from a centralized, experienced authority, and with time saved by the provision of labor-intensive services in a centralized facility.
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