CORE--CELL ISOLATION AND CULTURE

核心——细胞分离与培养

• 批准号: 6808274
• 负责人: GEORGE COOPER
• 金额: $ 9.95万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6808274
• 关键词: biomedical facility; heart cell; heart enlargement; intracardiac pressure; myocardium; pathologic process; tissue /cell culture; tissue /cell preparation

The majority of the program projects will utilize experimental preparations that require isolation and characterization of adult and neonatal cardiac muscle cells, or cardiocytes. This core will isolate cardiocytes from: 1) the adult cat, 2) the adult mouse, 3) the neonatal rat, and 4) the neonatal mouse. Adult cardiocytes will be isolated from both normal hearts and surgical models of pressure overload hypertrophy. These demands will best be met by a Core Facility where supply purchases, reagent preparation, and routine procedures can be performed with an economy based on scale, with quality control monitored from a centralized, experienced authority, and with time saved by the provision of labor-intensive services in a centralized facility.

大多数计划项目将利用需要分离和表征成人和新生儿心肌细胞或心肌细胞的实验准备。该核心将分离来自：1)成年猫，2)成年小鼠，3)新生大鼠和4)新生小鼠的心肌细胞。成人心肌细胞将从正常心脏和压力过载肥厚的手术模型中分离出来。这些需求最好由一个核心设施来满足，在那里，供应采购、试剂制备和常规程序可以以规模经济的方式进行，由一个有经验的中央权威机构进行质量控制，并通过在一个集中设施中提供劳动密集型服务来节省时间。