A massive attack on how messenger RNA sequence specifies condition-dependent RNA fate

对信使 RNA 序列如何指定条件依赖性 RNA 命运的大规模攻击

• 批准号: 2259690
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2259690
• 关键词: massive; attack; how; messenger; RNA

3'-untranslated regions (3'-UTRs) of mRNA are non-coding sequences that are involved in mRNA translation, localization and stability. However, the exact motifs involved in those functions remain largely unknown. The goal of this project is to understand the role of 3'-UTR sequences using model organism Saccharomyces cerevisiae.The project will use bioinformatics, transcriptomics and synthetic biology approaches to create thousands of chimeric mRNA sequences and to test them in multiple physiological and stress conditions, including measurements of mRNA production, processing, and decay. The obtained data can then be used to identify regulatory signals, for example sequence motifs in the untranslated regions of mRNAs and to create a coherent model of the function of 3'-UTR sequences in gene expression regulation and stress response.The results of this project will not only impact our understanding of stress response in budding yeast, but also increase our knowledge of post-transcriptional gene expression regulation. They might help us better understand and target pathogenic fungi species or become a foundation for similar studies, including mammalian development and pathology, as well as fine-tune protein expression in budding yeast for pharmaceutical purposes.

mRNA的3'-非翻译区域（3'-UTR）是与mRNA翻译，定位和稳定性有关的非编码序列。但是，这些功能所涉及的确切基序基本上仍然未知。该项目的目的是使用模型有机体酿酒酵母了解3'-UTR序列的作用。该项目将使用生物信息学，转录组学和合成生物学方法来创建数千种嵌合mRNA序列，并在多种生理和压力条件下测试它们，包括MRNA生产，包括MRNA生产，加工和DECAY。然后，获得的数据可用于识别调节信号，例如在未翻译的mRNA区域中的序列基序，并创建一个在基因表达调节和压力调节中的3'-UTR序列功能的连贯模型。该项目的结果不仅会影响我们对萌芽酵母中压力反应的理解，还会增加我们对后表达的认识。它们可能有助于我们更好地理解和靶向致病性真菌，或者成为类似研究的基础，包括哺乳动物的发育和病理，以及出于药物目的出现的酵母中的微调蛋白表达。