Genome Architecture in Human Sperm Cells

人类精子细胞的基因组结构

• 批准号: 6643507
• 负责人: ANDREI O ZALENSKY
• 金额: $ 16.71万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-08 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643507
• 关键词: DNA replication; cell cycle; cell nucleus; centromere; chromatin; chromosome movement; dimer; fertility; fluorescent in situ hybridization; gene rearrangement; genome; human tissue; in vitro fertilization; male; meiosis; microscopy; morphology; nucleic acid probes; nucleic acid quantitation /detection; nucleic acid structure; posttranscriptional RNA processing; semen; sperm; sperm analysis; telomere; tissue /cell preparation

The mammalian spermatozoa is a unique cell type in which the DNA is arranged into a compact and genetically inert state. Recently, the existence of a well-defined spatial organization of genome - genome architecture (GA) have been shown in spermatozoa of humans: each chromosome occupies distinct territory, all centromeres are clustered into a compact chromocenter buried within the nucleus, while all telomeres are exposed at the periphery and interact to form dimers. The general hypothesis is that non-random sperm GA determines and promotes an ordered chromosome withdrawal and movements during successful fertilization. In particular, defective GA in some cases of (male infertility may have an adverse impact on early development. The long-term goal is to establish the "standard" of how chromosomes are packed and arranged in normal, fertile human sperm, and to develop in situ tests for proper selection of donor cells during assisted reproduction. The first objective is to unravel as yet unknown characteristics of GA in sperm of donors with proven fertility. In preliminary experiments a new approach to directly observe chromosome organization in sperm has been developed. In the proposed study, nuclear path and higher- order structures of individual chromosomes will be established. Next, the nature of telomere dimers will be analyzed. Preliminary data indicate that these are formed by interactions between two ends of one chromosome. If so, chromosomes in human sperm nuclei are looped. Further, nuclear positioning of individual chromosomes will be determined. Strong indications towards non-random spatial arrangement of chromosomes in sperm nuclei have been obtained in preliminary studies. The second objective is a systematic study of GA in spermatozoa of men with abnormal sperm morphology undergoing treatment with IVF/ICSI. These studies will establish how chromosomes are packed and arranged in fertile human sperm and will lead to a better understanding of the role played by the male genome spatial organization at fertilization. The information obtained should contribute to an improvement of diagnostic assays and better prediction of the outcome of assisted reproduction. Finally it will add to our basic knowledge about structural organization of chromosomes in human cells.

哺乳动物精子是一种独特的细胞类型，其中DNA排列成紧凑和遗传惰性状态。最近，在人类精子中已经显示了基因组的明确定义的空间组织-基因组结构（GA）的存在：每个染色体占据不同的区域，所有的着丝粒聚集成一个紧凑的染色中心，埋藏在细胞核内，而所有的端粒暴露在外围并相互作用形成二聚体。 一般的假设是，非随机精子GA决定和促进有序的染色体撤回和成功受精过程中的运动。 特别是，在某些男性不育症的情况下，GA缺陷可能对早期发育产生不利影响。 长期目标是建立染色体如何在正常的、有生育能力的人类精子中包装和排列的“标准”，并开发原位测试，以便在辅助生殖过程中正确选择供体细胞。 第一个目标是解开尚未被证实的生育能力的捐赠者的精子GA的特点。 在初步实验中，已经开发出一种直接观察精子染色体结构的新方法。 在这项研究中，将建立单个染色体的核路径和高级结构.接下来，将分析端粒二聚体的性质。初步数据表明，它们是由一条染色体两端之间的相互作用形成的。 如果是这样，人类精子核中的染色体是环状的。 此外，将确定单个染色体的核定位。 在初步研究中，已经获得了精子核中染色体非随机空间排列的强有力迹象。 第二个目的是系统研究接受IVF/ICSI治疗的精子形态异常的男性精子中的GA。这些研究将确定染色体是如何在可育的人类精子中包装和排列的，并将导致更好地了解男性基因组空间组织在受精中所起的作用。 所获得的信息应有助于改进诊断检测并更好地预测辅助生殖的结果。 最后，它将增加我们对人类细胞中染色体结构组织的基本知识。