Elucidation and application of lignin degradation pathways in bacteria

目前木质素降解代谢工程中的一个瓶颈是对氧化的木质素片段转化为原儿茶酸或邻苯二酚等单环芳香族物质的代谢途径缺乏了解，然后通过已知的芳香降解途径进行加工。本项目的目的是通过报告分析和基因文库的筛选来阐明这些途径，并将这些途径应用于木质素降解的代谢工程以产生新的生物产品。

A current bottleneck in metabolic engineering of lignin degradation is a poor understanding of the metabolic pathways by which oxidised lignin fragments are converted into monocyclic aromatics such as protocatechuic acid or catechol, which are then processed by known aromatic degradation pathways. The aim of this project is to elucidate these pathways, using reporter assays and screening of gene libraries, and to apply these pathways for metabolic engineering of lignin degradation to generate new bioproducts.