A systems biology approach to unravel mechanisms of pesticide resistance

揭示农药抗性机制的系统生物学方法

• 批准号: 2264963
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2264963
• 关键词: systems; biology; approach; unravel; mechanisms

Pesticide resistance is a worldwide concern and elucidating the underlying mechanisms is critical for food security and human health. Understanding the mechanisms underpinning the process of pesticide resistance is challenging because they develop over evolutionary time, are influenced by foodweb dynamics and regulated by a complex machinery that involves direct organisms and indirect microbiota response. A transgenerational systems biology approach is need to unravel this complexity.Here, we use the exceptional model arthropod, the waterflea Daphnia, to study longitudinal dynamics that led to pesticide resistance. Daphnia enjoys many of the characteristics of other arthropods, such as a short life cycle and externally laid embryos. These embryos can be propagated indefinitely in the laboratory via clonal reproduction, allowing the rearing of populations of isogenic individuals (clones) from a single genotype. Additionally, Daphnia is unique for its extended dormancy that spans decades or even centuries, making this model ideal to study the underpinnign evolutionary mechanisms that enable pesticide resistance. We will apply a systems biology approach to resurrected specimens of Daphnia transisitioning from pristine to perturbed enviroments by agricultural land-use. We will quantify the level of pesticides in the environment using mass spectrometry and the composition of the food web using environmental DNA. We will link these parameters to changes in Daphnia genome-wide transcriptional, metabolomic and gut microbiota dynamics, establishing causal associations among foodweb dynamics, genome-wide transcriptional and microbiota changes.

农药抗药性是一个全球性问题，阐明其内在机制对粮食安全和人类健康至关重要。了解农药抗性过程的基础机制是具有挑战性的，因为它们是在进化过程中发展起来的，受到食物网动态的影响，并受到涉及直接生物和间接微生物群反应的复杂机制的调节。一个跨世代的系统生物学的方法是需要解开这个复杂性。在这里，我们使用特殊的模式节肢动物，水蚤水蚤，研究纵向动态，导致农药抗性。水蚤具有其他节肢动物的许多特征，例如生命周期短和外部胚胎。这些胚胎可以通过克隆繁殖在实验室中无限繁殖，从而可以从单一基因型培育出同基因个体（克隆）群体。此外，水蚤因其长达数十年甚至数百年的长期休眠而独一无二，这使得该模型成为研究实现杀虫剂抗性的基础进化机制的理想模型。我们将应用系统生物学的方法复活标本的水蚤transitioning从原始到扰动环境的农业土地利用。我们将使用质谱法量化环境中农药的含量，并使用环境DNA量化食物网的组成。我们将把这些参数与水蚤全基因组转录、代谢组学和肠道微生物群动态的变化联系起来，建立食物网动态、全基因组转录和微生物群变化之间的因果关系。