Packard Biochip DNA Microarray System

Packard Biochip DNA 微阵列系统

• 批准号: 6440917
• 负责人: LUCIA Amelia PIRISI-CREEK
• 金额: $ 20万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440917
• 关键词: DNA; biomedical equipment purchase; microarray technology; molecular biology

DESCRIPTION (provided by applicant): This application is to request funds ($291,170) to purchase the instruments, related accessories, computer, and software necessary to build a complete system for the production and analysis of spotted DNA microarrays. These are the Packard Biochip Technologies BioChip Arrayer, the ScanArray 4000 scanner, and the Packard Mutiprobe II robotic sample handling system. The microarray facility will be housed at the University of South Carolina School of Medicine, as an integral part of the well-established shared Instrumentation Resource Facility (IRF). The IRF is a core research and teaching facility used by more than 100 faculty, staff and students from the USC School of Medicine, the affiliated Dorn VA and Palmetto Richland Memorial Hospital, as well as by members of the South Carolina Cancer Center. The microarray faculity will serve 11 major users, most of whom are recipients of NIH grants. Many more investigators have expressed an interest in the technology, and a list of minor users already includes six investigators, and it is growing. Virtually all users are currently funded by external, peer reviewed sources, including the American Cancer Society, the American Heart Association, the Department of Defense, etc The microarray facility will serve all University of South Carolina faculty, students and staff researchers who have a need for this state-of-the-art technology. In addition, as it is the case with all IRF resources, the facility will be available to all interested researchers at MUSC, Clemson University, and other state and regional institutions. While there are two Affymetrics gene-chip facilities at a relative close distance (about 100 miles from USC: one at MUSC and the other at the Medical College of Georgia) there are currently no spotted array facilities available in this area. The development of DNA arrays, and more recently of microarrays represents a milestone of molecular biology, probably comparable in impact to the invention of PCR. Coupled with the complete sequencing of the human genome and of those of other species, this technology is taking studies of gene expression and DNA polymorphism to an entirely different level. Alterations in gene expression patterns or DNA polymorphisms determine or profoundly influence all biological functions. Variations in gene expression are at the core of development, differentiation, normal cell physiology, and alterations of gene expression drive or influence virtually all pathologic processes. DNA array technologies provide rapid and cost-effective means of identifying gene expression and genetic variations. Ready access to this technology is badly needed at the University of South Carolina and will foster tremendous growth in the biomedical research programs of this University and neighboring institutions.

描述（由申请人提供）：此申请是为了申请资金 （$291，170）购买仪器、有关配件、电脑，以及 建立完整的生产和分析系统所需的软件 斑点DNA微阵列这是帕卡德生物芯片技术公司的生物芯片 阵列仪、ScanArray 4000扫描仪和Packard Mutiprobe II机器人 样品处理系统微阵列设施将设在 南卡罗来纳州医学院，作为一个不可分割的一部分， 共享仪器资源设施（IRF）。IRF是一个 核心研究和教学设施，由100多名教职员工使用， 来自南加州大学医学院、附属的多恩弗吉尼亚大学和棕榈大学的学生 里奇兰纪念医院，以及成员的南卡罗来纳州癌症 中心微阵列学院将为11个主要用户提供服务，其中大多数是 获得NIH赠款的人。更多的研究人员表示有兴趣 这项技术，和一个小用户的名单已经包括六名调查人员， 而且还在增长目前，几乎所有用户都是由外部、同行 审查来源，包括美国癌症协会，美国心脏 协会，国防部等微阵列设施将服务于 所有南卡罗来纳州大学的教师，学生和工作人员的研究人员谁 需要这种最先进的技术。此外，由于 如果有国际公路联合会的所有资源，该设施将向所有感兴趣的人开放。 MUSC，克莱姆森大学和其他州和地区的研究人员 机构职能体系虽然有两个Affymetrics基因芯片设施， 相对近距离（距离南加州大学约100英里：一个在南加州大学，另一个在南加州大学） 格鲁吉亚医学院）目前没有点状阵列设施 在这个领域可用。DNA阵列的发展，以及最近的 微阵列代表了分子生物学的一个里程碑， 对PCR发明的影响。再加上完整的测序 人类基因组和其他物种的基因组，这项技术正在研究 基因表达和DNA多态性的水平。 基因表达模式或DNA多态性的改变决定或 影响着所有的生物功能。基因表达变异 是发育、分化、正常细胞生理学的核心， 基因表达的改变驱动或影响几乎所有的病理学改变， 流程. DNA阵列技术提供了快速和具有成本效益的手段， 识别基因表达和遗传变异。准备好进入这个 南卡罗来纳州大学迫切需要技术， 这所大学的生物医学研究项目的巨大增长， 邻近的机构。