LC/quadrupole ion trap mass spectrometer

LC/四极杆离子阱质谱仪

• 批准号: 6441125
• 负责人: BARRY M WILLARDSON
• 金额: $ 24.59万
• 依托单位: BRIGHAM YOUNG UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441125
• 关键词: AIDS; DNA binding protein; RNA binding protein; angiotensin receptor; apoptosis; biological signal transduction; biomedical equipment purchase; calmodulin dependent protein kinase; carcinogenesis; dendritic cells; electrospray ionization mass spectrometry; enzyme substrate; gas chromatography mass spectrometry; liquid chromatography mass spectrometry; mass spectrometry; molecular site; noninsulin dependent diabetes mellitus; nonvisual photoreceptor; pathologic process; phosphorylation; posttranslational modifications; prostaglandin endoperoxide synthase; protein structure function; receptor binding; receptor expression; transcription factor

The elucidation of covalent protein structure is a valuable tool in every field of biomedical research. This goal, the identification of protein sequence and post-translational modifications, is uniquely facilitated through mass spectrometry. Among mass spectrometers designed to analyze biological samples, quadruple ion trap instruments offer superior sensitivity and high quality fragmentation data, as well as coupling to liquid chromatography. For these reasons, we propose to obtain a LCQ DECA investigators from across campus will make use of this instrument, including a Major Users Group consisting of seven NIH RO1 grantees. The instrument will be used in the following ongoing funded research: Dr. BM Willardson will identity phosphorylation sites, interacting proteins, and transcription factors in his investigations of the roles of the phosducin family of G-protein regulators in modulating signal transduction. Dr. MB Andrus will identify receptor-binding drug candidates from solution-phase natural product-like libraries synthesized in his lab. Dr. TS Elton will identify both DNA and RNA binding proteins that play roles in regulating the expression of the angiotensin receptor. Dr. PB Savage will identify and characterize Lipid A-binding anti-microbial candidates from combinatorial libraries of cationic steroid triamides synthesized in his lab. Dr. DL Simmons will investigate the post-translational modifications of cyclooxygenase 2 and nucleobindin, as part of his research into the roles of these proteins in carcinogenesis and apoptosis. Dr. GF Burton will identify proteins implicated in the function of follicular dendritic cells in AIDS pathogenesis. And Dr. WW Winder will identify post-translational modifications, kinase targets, and transcription factors as part of his ongoing research into the physiology of exercise and type 2 diabetes. The LCQ mass spectrometer enables these investigations which would not be feasible otherwise.

蛋白质共价结构的解析在生物医学研究的各个领域都是一个有价值的工具。这一目标，蛋白质序列和翻译后修饰的鉴定，是独特的通过质谱促进。在设计用于分析生物样品的质谱仪中，四重离子阱仪器提供了上级灵敏度和高质量的碎片数据，以及与液相色谱的耦合。出于这些原因，我们建议获得一个LCQ DECA调查员从整个校园将利用这一工具，包括一个主要用户组组成的七个NIH RO1受赠者。该仪器将用于以下正在进行的资助研究：BM Willardson博士将在他对G蛋白调节剂的Euducin家族在调节信号转导中的作用的研究中识别磷酸化位点，相互作用的蛋白质和转录因子。MB Andrus博士将从他的实验室合成的液相天然产物样文库中鉴定受体结合药物候选物。TS Elton博士将鉴定在调节血管紧张素受体表达中发挥作用的DNA和RNA结合蛋白。PB Savage博士将从其实验室合成的阳离子类固醇三酰胺组合库中鉴定和表征脂质A结合的抗微生物候选物。DL西蒙斯博士将研究环氧合酶2和核结合蛋白的翻译后修饰，作为他研究这些蛋白质在致癌和凋亡中作用的一部分。GF Burton博士将鉴定与艾滋病发病机制中滤泡树突状细胞功能有关的蛋白质。WW Winder博士将确定翻译后修饰，激酶靶点和转录因子，作为他正在进行的运动和2型糖尿病生理学研究的一部分。LCQ质谱仪使这些研究成为可能，否则是不可行的。