Delta Vision Microscope Model 283 Configuration System

描述(申请人提供)：本共享设备建议书带来 来自多个部门的12名调查人员组成的不同小组聚集在一起， 西北大学医学院。该组织致力于广泛的 包括病毒(Longeck)、细菌(Seifert)原生动物的生物体 (Engman和Haldar)和哺乳动物细胞(Borise.格林、雷迪、米勒)。他们的 研究包括不同的细胞过程，从抗原到 加工、T细胞活化、细胞迁移、连接形成、细胞 病原体和新的真核有机体的分裂、黏附和入侵。 该小组的重点是首先检查蛋白质的表达、组织和 在活的和固定的细胞/组织中运输，然后与蛋白质相关 运输和信号传递的细胞事件的表达和分布， 受原核生物、低等真核生物和哺乳动物细胞的调控。这个 描述的应用是在宿主-病原体相互作用，哺乳动物细胞 生物学、运动学、免疫学。DeltaVision显微镜将使 调查人员必须：1)同时获取三重标记 样品；2)Low的采光系统的光学效率更高 强度样品；3)提高分辨率(共焦150 nm对200 nm) 用于研究细胞结构的细节；4)DIC；以及5)细胞核的可视化 用紫外线染料。这台显微镜可以完成所有不同的实验 集团的要求。对于所有八名调查人员来说，用一种 “租用？DeltaVision显微镜或在其他地方使用类似的显微镜 已经产生了重要的新的细胞洞察力。显微镜 将位于现有成像设施的中心，该成像设施具有 建立了培训和技术支持计划，并使用了70多个PI (以及更多的附属研究学院、博士后 研究员、研究生和研究助理)来自20个基础科学和 医学院的临床科室。DeitaVision显微镜租赁 已经被44个用户使用，表明了它的需求。购买该公司 DeitaVision显微镜将提供最先进的？显微镜可以是 适用于研究广泛的应用。它还将增强 在不同领域工作的研究人员之间的科学互动 生物学和人类健康在学校。

DESCRIPTION (provided by applicant): This shared equipment proposal brings together a diverse group of twelve investigators from multiple Departments at Northwestern University Medical School. The group works on a wide range of organisms that include viruses (Longnecker), bacteria (Seifert) protozoa (Engman and Haldar) and mammalian cells (Borisy. Green, Reddy, Miller). Their studies encompass diverse cellular processes that range from antigen processing, T cell activation, cell migration, junction formation, cell division, adhesion and invasion of pathogens and novel eukaryotic organdlies. The focus of the group is to first examine protein expression, organization and transport in both living and fixed cells/tissues and then correlate protein expression and distributions with cellular events of transport and signaling, as regulated by prokaryotes, lower eukaryotes and mammalian cells. The applications described are in Host-pathogen interactions, Mammalian Cell Biology, Motility, Immunology. The DeltaVision microscope will enable investigators to have : 1) simultaneous acquisition of triple-labeled specimens; 2) higher optical efficiency for light collection system for low intensity specimens; 3) increased resolution (150 nm vs. 200 nm for confocal) for studying details of cell structure; 4) DIC; and 5) visualization of nuclei with UV dyes. This microscope fulfills all of the different experimental requirements of the group. For all eight investigators, experiments with a "leased? DeltaVision microscope or access to a similar microscope at other universities, have resulted in important new cellular insights. The microscope will be centrally located in an existing Imaging Facility that has well established training and technical support programs, and is used over 70 PIs (and an even larger number of affiliated Research Faculty, Postdoctoral Fellows, Graduate Students and Research Associates) from 20 basic science and clinical departments in the Medical School. A DeitaVision microscope on lease has been used by 44 users indicating its demand. The purchase of the DeitaVision microscope will provide a ?state-of-the-art? microscope that can be applied to studying a wide range of applications. It will also enhance scientific interactions between investigators working in diverse areas of biology and human health in the school.