Acquisition of DNA Sequencing Capability

获得DNA测序能力

• 批准号: 6440905
• 负责人: Sarjeet S Gill
• 金额: $ 29.29万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440905
• 关键词: Aedes; Culicidae; arthropod genetics; biomedical equipment purchase; complementary DNA; computer assisted sequence analysis; cytogenetics; gene expression; gene mutation; genetic polymorphism; genetic regulation; genetic transcription; microarray technology; nucleic acid sequence; protein tyrosine kinase

A capillary DNA sequencer and associated instruments are requested to establish a core DNA sequencing facility at the University of California, Riverside. The principal investigator and seven other major users, all with NIH funding will need increased sequencing capability to identify DNA isolated in their research programs. The campus presently does not have any DNA sequencing facility where these investigators have ready access with reasonable costs. These investigators are involved in health- related research that include elucidation of biochemical processes in human disease bearing mosquitoes and their transformation, liver specific gene expression radiation induced mutagenesis and genomic instability, regulation of p53-mediated transcription, role of receptor tyrosine kinases and cell cycle genes in the shear stress-induced activation. These users wish to identify development of EST database for mosquitoes, DNA microarray development, mutation analysis, polymorphisms, determination of cDNAs and genes, and confirmation of expression constructs. All of the NIH funded grants would benefit from a core DNA facility on campus. The facility would also be of help for other investigators with NSL and USDA funding to elucidate sex- determination genes, identifying genes involved in sporulation and infection cycles in fungi, genes involved in plant defense mechanisms, and genetics of abiotic stress tolerance.

加州大学河滨分校需要配备毛细管 DNA 测序仪和相关仪器来建立核心 DNA 测序设施。首席研究员和其他七位主要用户都获得了 NIH 的资助，他们需要增强测序能力来识别在其研究项目中分离的 DNA。该校园目前没有任何 DNA 测序设施，这些调查人员可以以合理的成本使用这些设施。这些研究人员参与与健康相关的研究，包括阐明人类携带疾病的蚊子的生化过程及其转化、肝脏特异性基因表达辐射诱导的突变和基因组不稳定性、p53介导的转录的调节、受体酪氨酸激酶和细胞周期基因在剪切应力诱导的激活中的作用。这些用户希望确定蚊子 EST 数据库的开发、DNA 微阵列开发、突变分析、多态性、cDNA 和基因的测定以及表达构建体的确认。 NIH 资助的所有拨款都将受益于校园内的核心 DNA 设施。该设施还将帮助其他研究人员在国家实验室和美国农业部的资助下阐明性别决定基因、鉴定真菌中孢子形成和感染周期所涉及的基因、植物防御机制所涉及的基因以及非生物胁迫耐受性的遗传学。