Acquisition of DNA Sequencing Capability

需要毛细管DNA测序仪和相关仪器在加州大学河滨分校建立一个核心DNA测序设施。首席研究员和其他七个主要用户，都是由NIH资助的，将需要提高测序能力，以识别在他们的研究项目中分离的DNA。校园目前没有任何DNA测序设备，这些研究人员可以以合理的成本随时访问。这些研究人员从事与健康相关的研究，包括阐明携带人类疾病的蚊子的生化过程及其转化，肝脏特异性基因表达辐射诱导的诱变和基因组不稳定性，p53介导的转录调节，受体酪氨酸激酶和细胞周期基因在剪切应力诱导的激活中的作用。这些用户希望确定蚊子EST数据库的开发、DNA微阵列的开发、突变分析、多态性、cdna和基因的测定以及表达结构的确认。美国国立卫生研究院资助的所有资金都将从校园内的核心DNA设施中受益。该设施还将帮助美国国家自然科学实验室和美国农业部资助的其他研究人员阐明性别决定基因，鉴定与真菌孢子形成和感染周期有关的基因，与植物防御机制有关的基因，以及非生物胁迫耐受性的遗传。

A capillary DNA sequencer and associated instruments are requested to establish a core DNA sequencing facility at the University of California, Riverside. The principal investigator and seven other major users, all with NIH funding will need increased sequencing capability to identify DNA isolated in their research programs. The campus presently does not have any DNA sequencing facility where these investigators have ready access with reasonable costs. These investigators are involved in health- related research that include elucidation of biochemical processes in human disease bearing mosquitoes and their transformation, liver specific gene expression radiation induced mutagenesis and genomic instability, regulation of p53-mediated transcription, role of receptor tyrosine kinases and cell cycle genes in the shear stress-induced activation. These users wish to identify development of EST database for mosquitoes, DNA microarray development, mutation analysis, polymorphisms, determination of cDNAs and genes, and confirmation of expression constructs. All of the NIH funded grants would benefit from a core DNA facility on campus. The facility would also be of help for other investigators with NSL and USDA funding to elucidate sex- determination genes, identifying genes involved in sporulation and infection cycles in fungi, genes involved in plant defense mechanisms, and genetics of abiotic stress tolerance.