Acquisition of DNA Sequencing Capability

一个毛细管DNA测序仪和相关的仪器被要求在加州大学滨江分校建立一个核心DNA测序设施。首席研究员和其他七个主要用户，都与美国国立卫生研究院的资金将需要增加测序能力，以确定DNA分离在他们的研究计划。校园目前没有任何DNA测序设施，这些调查人员可以以合理的费用随时访问。这些研究人员参与了与健康相关的研究，包括阐明携带人类疾病的蚊子及其转化的生化过程、肝脏特异性基因表达辐射诱导的诱变和基因组不稳定性、p53介导的转录调控、受体酪氨酸激酶和细胞周期基因在剪切应力诱导的激活中的作用。这些用户希望确定蚊子EST数据库的开发、DNA微阵列的开发、突变分析、多态性、cDNA和基因的测定以及表达构建体的确认。所有NIH资助的赠款都将受益于校园内的核心DNA设施。该设施也将有助于其他研究人员与NSL和美国农业部的资金，以阐明性别决定基因，确定基因参与孢子形成和感染周期的真菌，基因参与植物防御机制，和遗传学的非生物胁迫耐受性。

A capillary DNA sequencer and associated instruments are requested to establish a core DNA sequencing facility at the University of California, Riverside. The principal investigator and seven other major users, all with NIH funding will need increased sequencing capability to identify DNA isolated in their research programs. The campus presently does not have any DNA sequencing facility where these investigators have ready access with reasonable costs. These investigators are involved in health- related research that include elucidation of biochemical processes in human disease bearing mosquitoes and their transformation, liver specific gene expression radiation induced mutagenesis and genomic instability, regulation of p53-mediated transcription, role of receptor tyrosine kinases and cell cycle genes in the shear stress-induced activation. These users wish to identify development of EST database for mosquitoes, DNA microarray development, mutation analysis, polymorphisms, determination of cDNAs and genes, and confirmation of expression constructs. All of the NIH funded grants would benefit from a core DNA facility on campus. The facility would also be of help for other investigators with NSL and USDA funding to elucidate sex- determination genes, identifying genes involved in sporulation and infection cycles in fungi, genes involved in plant defense mechanisms, and genetics of abiotic stress tolerance.