MEMBRANE TRANSPORTERS IN MOSQUITOES

描述（改编自申请人的摘要）：这笔赠款的目标是 PI 的长期关注点，以表征结构和功能 蚊子的中肠。这笔资助的重点是阐明分子 涉及埃及伊蚊营养吸收的过程。通过分子 PI 已分离出识别中肠转运过程的方法 3 个氨基酸转运蛋白的 cDNA，这是形成氨基酸转运蛋白所需的潜在蛋白质 具有氨基酸转运蛋白的功能性异二聚体和假定的蔗糖 来自埃及伊蚊中肠的转运蛋白。在目标 1 中，PI 建议 继续分离其中两种蛋白质的全长 cDNA 克隆 他有部分 cDNA。这些 cDNA 将在异源表达 表达系统（非洲爪蟾卵母细胞）和该系统用于表征 每种氨基酸转运蛋白和离子的底物选择性 运输过程的选择性。在第二个目标中，类似 将对蔗糖转运蛋白进行实验。作为决赛 目的，将分析这些转运蛋白的体内调节。的 将在体内分析基因的表达以确定是否存在 糖或血液后的发育调节和调节 喂养。

DESCRIPTION (Adapted From The Applicant's Abstract): This grant is targeted towards the PI's long-term focus to characterize the structure and function of the midgut of mosquitoes. This grant focuses upon elucidating the molecular processes involved in nutrient uptake in Aedes aegypti. Through a molecular approach to identify transport processes at the midgut, the PI has isolated cDNA's for 3 amino-acid transporters, a potential protein needed to form a functional heterodimer with the amino acid transporters, and a putative sucrose transporter from the midgut of Aedes aegypti. In objective 1, the PI proposes to continue in the isolation of full-length cDNA clones for two of the proteins for which he has partial cDNAs. These cDNAs will be expressed in a heterologous expression system (Xenopus oocytes) and this system used to characterize the substrate selectivity of each of the amino acid transporters and the ion selectivity of the transport process. In the second objective, similar experiments will be performed for the sucrose transporter. As the final objective, the in vivo regulation of these transporters will be analyzed. The expression of the genes will be analyzed in vivo to determine if there is developmental regulation and modulation following either sugar or blood feeding.