MEMBRANE TRANSPORTERS IN MOSQUITOES

描述(改编自申请者摘要)：这笔赠款是有针对性的 对于PI的长期关注，以表征其结构和功能 蚊子的中肠。这项资助的重点是阐明分子 埃及伊蚊营养吸收过程的研究。通过一种分子 确定中肠转运过程的方法，PI分离出 3个氨基酸转运体的cDNA，一个潜在的蛋白质，需要形成一个 带有氨基酸转运体的功能杂二聚体，以及可能的蔗糖 埃及伊蚊中肠的转运蛋白。在目标1中，国际和平倡议提出 继续分离其中两种蛋白质的全长cdna克隆 他有部分的DNA。这些cDNA将在一个异源的 表达系统(非洲爪哇卵母细胞)，该系统用于表征 每种氨基酸转运体和离子的底物选择性 运输过程的选择性。在第二个目标中，类似 将对蔗糖转运体进行实验。作为决赛 目的：分析这些转运蛋白在体内的调控情况。这个 这些基因的表达将在体内进行分析，以确定是否存在 糖或血对发育的调节和调节 喂食。

DESCRIPTION (Adapted From The Applicant's Abstract): This grant is targeted towards the PI's long-term focus to characterize the structure and function of the midgut of mosquitoes. This grant focuses upon elucidating the molecular processes involved in nutrient uptake in Aedes aegypti. Through a molecular approach to identify transport processes at the midgut, the PI has isolated cDNA's for 3 amino-acid transporters, a potential protein needed to form a functional heterodimer with the amino acid transporters, and a putative sucrose transporter from the midgut of Aedes aegypti. In objective 1, the PI proposes to continue in the isolation of full-length cDNA clones for two of the proteins for which he has partial cDNAs. These cDNAs will be expressed in a heterologous expression system (Xenopus oocytes) and this system used to characterize the substrate selectivity of each of the amino acid transporters and the ion selectivity of the transport process. In the second objective, similar experiments will be performed for the sucrose transporter. As the final objective, the in vivo regulation of these transporters will be analyzed. The expression of the genes will be analyzed in vivo to determine if there is developmental regulation and modulation following either sugar or blood feeding.