Fluorescence, Chemiluminescence and Phosphor Imager

荧光、化学发光和磷光成像仪

• 批准号: 6440395
• 负责人: MICHAEL T BERTON
• 金额: $ 12.36万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440395
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; digital imaging; fluorescence; luminescence; phosphorescence; radionuclides

A molecular Dynamics Typhoon 8600 variable model gel and blot imaging system is requested to support the ongoing and future studies of researchers at the University of Texas Health Science Center at San Antonio (UTHSCSA). This instrument will support research that requires state-of-the-art image analysis instrumentation to study molecular and cellular function in a diverse array of research projects devoted to understanding fundamental processes in human disease. The proposed instrument will greatly enhance the research of nine major users from four different departments from the Medical and Graduate Schools. Common features of this diverse group of users include: 1) an immediate need for on site fluorescent, chemiluminescent and storage phosphor imaging capabilities, 2) research projects committed to understanding fundamental biological questions relevant to human disease, and 3) NIH-funded research programs that have specific needs for rapid, sensitive and flexible digital imaging of radioactive, fluorescent and chemiluminescent samples. Research in the following specific aims will be significantly enhanced by the proposed instrument: 1) Immunoglobulin class switching, 2) Trichomonas vaginalis pathogenesis, 3) NADPH oxidases and inflammation, 4) Salmonella pathogenesis and anti-microbial peptides, 5) Vibrio Cholerae pathogenesis, 6( Insulin receptor signaling, 7) Neonatal respiratory distress syndrome (Mustafa), 8) B cell development and parasite immunity, and 9) Genetics of virulence in pathogenic fungi. The Typhoon 8600 has the strong endorsement of the institution. The University will provide laboratory space and resources for instrument maintenance and service and the instrument will be an essential component of the Nucleic Acids Technology Core facility. This core currently offers DNA sequencing with data analysis and primer design, custom DNA/RNA synthesis, gene amplification services, and robotic applications. The addition of the Typhoon will significantly enhance the capabilities of this core facility and provide great benefit to research programs at UTHSCSA and in South Texas.

需要使用分子动力学 Typhoon 8600 可变模型凝胶和印迹成像系统来支持圣安东尼奥德克萨斯大学健康科学中心 (UTHSCSA) 研究人员正在进行和未来的研究。该仪器将支持需要最先进的图像分析仪器来研究各种研究项目中的分子和细胞功能的研究，这些研究项目致力于了解人类疾病的基本过程。拟议的仪器将极大地增强来自医学院和研究生院四个不同部门的九个主要用户的研究。这一多样化用户群体的共同特征包括：1) 对现场荧光、化学发光和存储荧光体成像功能的迫切需求，2) 致力于了解与人类疾病相关的基本生物学问题的研究项目，以及 3) NIH 资助的研究项目，这些项目对放射性、荧光和化学发光样品的快速、灵敏和灵活的数字成像有特定的需求。该仪器将显着增强以下具体目标的研究：1) 免疫球蛋白类别转换，2) 阴道毛滴虫发病机制，3) NADPH 氧化酶和炎症，4) 沙门氏菌发病机制和抗菌肽，5) 霍乱弧菌发病机制，6( 胰岛素受体信号传导，7) 新生儿呼吸窘迫综合征 (Mustafa)，8) B 细胞发育和寄生虫免疫，以及 9) 病原真菌毒力遗传学。台风8600得到了机构的大力认可。该大学将为仪器维护和服务提供实验室空间和资源，该仪器将成为核酸技术核心设施的重要组成部分。该核心目前提供 DNA 测序、数据分析和引物设计、定制 DNA/RNA 合成、基因扩增服务和机器人应用。台风的加入将显着增强该核心设施的能力，并为 UTHSCSA 和南德克萨斯州的研究项目带来巨大好处。