权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mays Cancer Center at UT Health SA

UT Health SA 梅斯癌症中心

基本信息

批准号：
10237954
负责人：
MICHAEL T BERTON
金额：
$ 7.91万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10237954
关键词：
Adoption Advisory Committees Applications Grants Area Award Budgets Cancer Center Caspase Cell Cycle Cell Separation Cells Clone Cells Computer Analysis Computer Workstations Computer software Consultations Cytometry Data Data Analyses Doctor of Philosophy Dyes Equipment Experimental Designs Flow Cytometry Flow Cytometry Shared Resource Funding Genomics Grant Group Meetings Health Laboratories Malignant Neoplasms Manuscripts Measurement Methods Microbiology Mission Mitochondria Molecular Genetics Molecular Immunology Molecular Medicine Names Nitrogen Oxygen Peer Review Preparation Production Productivity Publications Reporting Research Research Personnel Resource Sharing Series Services Sorting - Cell Movement Supervision Technical Expertise Technology Texas Time Training Training Programs Training and Education Universities Writing certificate program cost effective equipment acquisition high standard improved instrument member mid-career faculty new technology operation programs square foot transcriptomics

项目摘要

Project Summary—Flow Cytometry Shared Resource The Flow Cytometry Shared Resource (FCSR) has been a Cancer Center-supported Shared Resource since 1991. The mission of the FCSR is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for Mays Cancer Center (MCC) members, University of Texas Health San Antonio (UT Health SA) investigators, and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible by MCC members. FCSR services include multiparameter analysis and sorting of subpopulations of cells; cell cycle analysis; single cell cloning and sorting for downstream genomics/transcriptomics; measurements of reactive nitrogen and oxygen species (NOS and ROS), pH, Ca++ fluxes and fluxes of different vital dyes; study of mitochondrial damage; and determination of activated caspases. The FCSR also offers consultative services including experimental design, instrument training, and assistance with grant and manuscript writing (including preparation of figures, methods sections, and budgets). The FCSR provides MCC members access to two flow cytometry cell sorters, four conventional analyzers, a spectral analyzer and, most recently, a mass cytometer. The FCSR is led by Scientific Director Michael Berton, Ph.D., Associate Professor of Microbiology, Immunology & Molecular Genetics. Ms. Karla Gorena is a Technical Director and daily operations manager of the FCSR. Nameer Kirma, Ph.D., Associate Professor of Molecular Medicine, joined the FCSR as a second Technical Director in 2018 to supervise the Helios mass cytometer. The FCSR is administratively supported by the UT Health SA Office of the Vice President for Research (VPR) and the MCC. During 2018, 35 MCC members with peer-reviewed funding used the FCSR. In addition, the FCSR contributed to 53 cancer-related publications during the last reporting period (2014-2018).

项目概述-流式细胞术共享资源