FACSAria Fusion cell sorter for Flow Cytometry Shared Resource

项目总结/摘要 位于圣安东尼奥的德克萨斯大学健康科学中心的使命 （FCSR）是使用最先进的设备提供高通量、多维分析和细胞分选 为UT健康圣安东尼奥调查人员和其他在圣安东尼奥地区。FCSR占地800多平方米。 英尺实验室的空间，并在校园的中心方便访问。FCSR完成了其 使命，允许获得质量控制，高度专业化的技术，教育和技术 以具有成本效益的方式提供援助。整个园区的所有分拣需求都在两个BD上完成 仪器，其中之一是在个人PI实验室和机构用户不可用。一个分拣机 在FCSR中，尽管最大的实际使用时间是1500小时， 容量约为1300小时。当前分拣机的这种过度利用， 由于其不可靠性，这台已有17年历史的仪器的需求和停机时间不断增加， 样品和调度等待时间为2-3周。此外，我们的一些用户正在进行独特的高度排序， 来自人类患者的有价值的样本，可在短时间内获得，数量有限。 由于分拣机等待时间长， 当前分拣机的频繁停机。此外，当前分拣机的性能和能力 不足以满足对更高参数细胞分选的日益增长的需求。买了一把5激光40- 参数Cytek Aurora全谱细胞分析仪在2019年6月，我们的许多用户已经过渡或正在 在过渡到更复杂的>15个参数分析的过程中。目前的BD FACSAria IIIu缺乏 将这些面板转化为细胞分选以用于进一步下游测定的能力，因此限制了对 我们的许多最频繁的用户。本提案用于购买BD FACSAria融合细胞分选仪， 五个激光器（355，405，488，561和637 nm）能够分析多达18种不同的颜色， 侧面散射。该仪器将为FCSR提供迫切需要的额外分选能力， 还提供高参数细胞分选和更好地检测和分选稀有细胞群的能力。 我们还需要可选的集成Baker Class II Type A2生物安全罩和集成气雾剂 管理系统，以适应BSL-2安全壳的分拣。

Project Summary/Abstract The mission of the University of Texas Health Science Center at San Antonio Flow Cytometry Shared Resource (FCSR) is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for UT Health San Antonio investigators and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible in the center of campus. The FCSR accomplishes its mission by allowing access to quality-controlled, highly specialized technology, education and technical assistance in a cost-effective manner. All sorting demands for the entire campus are accomplished on two BD instruments, one of which is in an individual PI laboratory and unavailable to institutional users. The one sorter in the FCSR is heavily utilized and exceeds over 1500 hours of annual use, even though the maximum practical capacity is approximately 1300 hours. This overutilization of the current sorter coupled with the increasing demand and increasing downtime of this 17-year-old instrument due to its unreliability, leads to loss of valuable samples and scheduling wait times of 2-3 weeks. In addition, a number of our users are sorting unique and highly valued samples from human patients that become available on short notice and are in limited quantity. Accommodating these users has become increasingly difficult due to the long wait times for sorter time and the frequent downtime for the current sorter. Additionally, the performance and capabilities of the current sorter are not sufficient to meet the increasing demand for higher parameter cell sorting. With the purchase of a 5-laser 40- parameter Cytek Aurora full spectrum cell analyzer in June of 2019, many of our users have transitioned or are in the process of transitioning to more complex >15-parameter analyses. The current BD FACSAria IIIu lacks the ability to translate these panels into cell sorting for further downstream assays, thus limiting the research of many of our most frequent users. This proposal is for purchase of a BD FACSAria Fusion cell sorter equipped with five lasers (355, 405, 488, 561, and 637nm) capable of analyzing up to 18 different colors plus Forward and Side Scatter. The instrument will provide desperately needed additional sorting capacity for the FCSR and will also provide the capability for high parameter cell sorting and better detection and sorting of rare cell populations. We are also requesting the optional integrated Baker Class II Type A2 biosafety hood and integrated Aerosol Management System to accommodate sorting at BSL-2 containment.