FACSAria Fusion cell sorter for Flow Cytometry Shared Resource

项目摘要/摘要 位于圣安东尼奥的德克萨斯大学健康科学中心的使命--流式细胞术共享资源 (FCSR)是使用最先进的设备提供高通量、多维分析和细胞分选 对于德克萨斯大学健康圣安东尼奥分校的调查人员和圣安东尼奥地区的其他人。FCSR占地800多平方米。 英国《金融时报》是实验室空间的一部分，在校园中心可以方便地到达。FCSR完成了其 特派团，允许获得质量受控、高度专业化的技术、教育和技术 以具有成本效益的方式提供援助。整个园区的所有分拣要求都在两个BD上完成 仪器，其中一个在单独的PI实验室，机构用户无法获得。唯一的分拣员 在FCSR中的使用率很高，每年超过1500小时，尽管最大实际使用时间 载客量约为1300小时。这种对当前分拣机的过度使用，加上不断增加的 这台已有17年历史的仪器由于不可靠，需求和停机时间不断增加，导致宝贵的损失 样品和排料的等待时间为2-3周。此外，我们的一些用户正在对独特的和高度的 来自人类患者的有价值的样本，可在短时间内获得，并且数量有限。 由于分拣时间的等待时间很长，以及 当前分拣机频繁停机。此外，当前排序器的性能和功能是 不足以满足日益增长的对更高参数的单元格排序的需求。购买了一台5-激光40- 参数Cytek Aurora全频谱细胞分析仪在2019年6月，我们的许多用户已经过渡或正在 在向更复杂的15个参数分析过渡的过程中。目前的BD FACSARia IIIu缺乏 将这些面板转化为细胞分选以进行进一步下游分析的能力，从而限制了对 我们的许多最频繁的用户。这项建议是为了购买一台BD FACSAria融合细胞分选机 具有五种激光(355、405、488、561和637 nm)，能够分析多达18种不同的颜色，并向前和 侧向散布。该仪器将为FCSR提供迫切需要的额外分拣能力，并将 还提供高参数细胞分选以及更好地检测和分选稀有细胞群的能力。 我们还需要可选的集成面包师II类A2生物安全罩和集成气雾剂 管理系统，以适应BSL-2安全壳的分拣。

Project Summary/Abstract The mission of the University of Texas Health Science Center at San Antonio Flow Cytometry Shared Resource (FCSR) is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for UT Health San Antonio investigators and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible in the center of campus. The FCSR accomplishes its mission by allowing access to quality-controlled, highly specialized technology, education and technical assistance in a cost-effective manner. All sorting demands for the entire campus are accomplished on two BD instruments, one of which is in an individual PI laboratory and unavailable to institutional users. The one sorter in the FCSR is heavily utilized and exceeds over 1500 hours of annual use, even though the maximum practical capacity is approximately 1300 hours. This overutilization of the current sorter coupled with the increasing demand and increasing downtime of this 17-year-old instrument due to its unreliability, leads to loss of valuable samples and scheduling wait times of 2-3 weeks. In addition, a number of our users are sorting unique and highly valued samples from human patients that become available on short notice and are in limited quantity. Accommodating these users has become increasingly difficult due to the long wait times for sorter time and the frequent downtime for the current sorter. Additionally, the performance and capabilities of the current sorter are not sufficient to meet the increasing demand for higher parameter cell sorting. With the purchase of a 5-laser 40- parameter Cytek Aurora full spectrum cell analyzer in June of 2019, many of our users have transitioned or are in the process of transitioning to more complex >15-parameter analyses. The current BD FACSAria IIIu lacks the ability to translate these panels into cell sorting for further downstream assays, thus limiting the research of many of our most frequent users. This proposal is for purchase of a BD FACSAria Fusion cell sorter equipped with five lasers (355, 405, 488, 561, and 637nm) capable of analyzing up to 18 different colors plus Forward and Side Scatter. The instrument will provide desperately needed additional sorting capacity for the FCSR and will also provide the capability for high parameter cell sorting and better detection and sorting of rare cell populations. We are also requesting the optional integrated Baker Class II Type A2 biosafety hood and integrated Aerosol Management System to accommodate sorting at BSL-2 containment.