FACSAria Fusion cell sorter for Flow Cytometry Shared Resource

用于流式细胞术共享资源的 FACSAria Fusion 细胞分选仪

• 批准号: 10431534
• 负责人: MICHAEL T BERTON
• 金额: $ 59.6万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-15 至 2023-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431534
• 关键词: 17 year old; Aerosols; Area; Biological Assay; Cell Separation; Cell fusion; Cells; Color; Complex; Containment; Coupled; Detection; Education; Equipment; Flow Cytometry Shared Resource; Health; Health Sciences; Hour; Human; Individual; Laboratories; Lasers; Mission; Patients; Performance; Population; Process; Research; Research Personnel; Sampling; Schedule; Side; Sorting - Cell Movement; System; Technology; Texas; Time; Translating; Universities; Wait Time; cell analyzer; cost effective; instrument; square foot

Project Summary/Abstract The mission of the University of Texas Health Science Center at San Antonio Flow Cytometry Shared Resource (FCSR) is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for UT Health San Antonio investigators and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible in the center of campus. The FCSR accomplishes its mission by allowing access to quality-controlled, highly specialized technology, education and technical assistance in a cost-effective manner. All sorting demands for the entire campus are accomplished on two BD instruments, one of which is in an individual PI laboratory and unavailable to institutional users. The one sorter in the FCSR is heavily utilized and exceeds over 1500 hours of annual use, even though the maximum practical capacity is approximately 1300 hours. This overutilization of the current sorter coupled with the increasing demand and increasing downtime of this 17-year-old instrument due to its unreliability, leads to loss of valuable samples and scheduling wait times of 2-3 weeks. In addition, a number of our users are sorting unique and highly valued samples from human patients that become available on short notice and are in limited quantity. Accommodating these users has become increasingly difficult due to the long wait times for sorter time and the frequent downtime for the current sorter. Additionally, the performance and capabilities of the current sorter are not sufficient to meet the increasing demand for higher parameter cell sorting. With the purchase of a 5-laser 40- parameter Cytek Aurora full spectrum cell analyzer in June of 2019, many of our users have transitioned or are in the process of transitioning to more complex >15-parameter analyses. The current BD FACSAria IIIu lacks the ability to translate these panels into cell sorting for further downstream assays, thus limiting the research of many of our most frequent users. This proposal is for purchase of a BD FACSAria Fusion cell sorter equipped with five lasers (355, 405, 488, 561, and 637nm) capable of analyzing up to 18 different colors plus Forward and Side Scatter. The instrument will provide desperately needed additional sorting capacity for the FCSR and will also provide the capability for high parameter cell sorting and better detection and sorting of rare cell populations. We are also requesting the optional integrated Baker Class II Type A2 biosafety hood and integrated Aerosol Management System to accommodate sorting at BSL-2 containment.

项目概要/摘要 圣安东尼奥德克萨斯大学健康科学中心的使命流式细胞仪共享资源 (FCSR) 旨在使用最先进的设备提供高通量、多维分析和细胞分选 为 UT Health San Antonio 的调查人员和圣安东尼奥地区的其他人提供。 FCSR占地800多平方米。 实验室空间的英尺，位于校园中心，交通便利。 FCSR 完成了它的任务 通过允许获得质量控制的、高度专业化的技术、教育和技术来完成使命 以具有成本效益的方式提供援助。整个园区的所有分拣需求均在两个BD上完成 仪器，其中一台位于单个 PI 实验室，机构用户无法使用。一台分拣机 FCSR 中的利用率很高，每年使用时间超过 1500 小时，尽管最大实际使用时间 容量约为1300小时。当前分拣机的过度利用加上日益增加的 由于这种已有 17 年历史的仪器不可靠，其需求量和停机时间不断增加，导致宝贵的损失 样品和安排等待时间为 2-3 周。此外，我们的许多用户都在排序独特且高度 来自人类患者的宝贵样本可在短时间内提供，且数量有限。 由于分拣机的等待时间较长以及 当前分拣机频繁停机。此外，当前分拣机的性能和功能是 不足以满足对更高参数细胞分选不断增长的需求。购买 5 激光 40- 2019年6月参数Cytek Aurora全谱细胞分析仪，我们很多用户已经转型或者正在转型 正在过渡到更复杂的 >15 参数分析。目前的 BD FACSAria IIIu 缺乏 将这些面板转化为细胞分选以进行进一步下游分析的能力，从而限制了以下研究 我们许多最常使用的用户。本提案用于购买配备的 BD FACSAria Fusion 细胞分选机 具有五种激光器（355、405、488、561 和 637nm），能够分析多达 18 种不同的颜色以及正向和 侧向散射。该仪器将为 FCSR 提供​​急需的额外分选能力，并将 还提供高参数细胞分选以及更好地检测和分选稀有细胞群的能力。 我们还要求可选的集成 Baker Class II A2 型生物安全罩和集成气溶胶 用于在 BSL-2 安全壳内进行分类的管理系统。