EFFECTS OF CARBOPLATIN AND KAINIC ACID ON EVOKED RESPONSES

卡铂和红藻氨酸对诱发反应的影响

• 批准号: 6662127
• 负责人: ROBERT BURKARD
• 金额: $ 16.54万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-19 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6662127
• 关键词: action potentials; auditory threshold; carboplatin; chinchilla; denervation; ear hair cell; ear pharmacology; electrophysiology; evoked potentials; gamma aminobutyrate; kainate; neural information processing; neuropharmacology; neurotoxicology; neurotoxins; neurotransmitter antagonist; perceptual maskings; sensorineural hearing loss

A series of experiments are proposed which investigate the functional consequence of either permanent inner hair cell (IHC) loss using carboplatin or temporary degeneration of eight nerve fibers using kainic acid. The experimental animal will be the chinchilla. Preliminary data from this laboratory demonstrate different patters of changes in evoke potential amplitude at varying locations in the auditory nervous system following injection of carboplatin or application of kainic acid to the round window. We will survey outer hair cell integrity by recording distortion product otoacoustic emissions from the early canal of each animal. Following baseline studies, we will evaluate the time course of the functional consequence of carboplatin and kainic acid on the responses from the eighth nerve, inferior colliculus and auditory cortex. Using this approach, we will investigate the role of IHC function on auditory function and will study functional reorganization in the auditory system in response to permanent IHC loss and transient deafferentation. Several experiments will use GABA blockers to test the hypothesis that disinhibition plays a role in compensatory changes seen in central auditory structures following peripheral damage. A fundamental assumption of this work is that a dissociation of peripheral and central effects of ototoxic insults, or a differing time course of recovery is evidence of compensatory functional reorganization.

提出了一系列的实验，研究功能 永久性内毛细胞（IHC）损失的后果， 卡铂或暂时变性的八个神经纤维使用红藻氨酸 酸实验动物将是栗鼠。初步数据 从这个实验室展示了不同的模式的变化， 听觉神经系统中不同位置的电位幅度 注射卡铂或应用红藻氨酸后， 圆窗我们将调查外毛细胞的完整性， 畸变产物耳声发射从早期运河的每个 动物在基线研究之后，我们将评估 卡铂和红藻氨酸对反应的功能影响 第八神经下丘和听觉皮层使用此 方法，我们将研究IHC功能对听觉的作用， 功能，并将研究听觉系统的功能重组 对永久性IHC丧失和暂时性传入神经阻滞的反应。几 实验将使用GABA阻滞剂来检验这一假设， 去抑制在中枢神经系统的代偿性变化中起作用。 听觉结构周围损伤。一个基本假设 这项工作的一个重要特点是， 耳毒性损伤或不同的恢复时间过程是 补偿性功能重组