CORE--LABORATORY

UTI核心实验室将提供必要的细胞培养， 所有四个项目的微生物和分子分型程序 节目集中执行这些专门程序 确保其可用性、高质量和降低的成本。以下 将提供以下服务： (1)用于分离和表征的微生物程序 E.大肠杆菌和乳酸杆菌在项目1和3。 (2)培养（a）原代移行上皮细胞用于 细菌鞘糖脂合成调节的研究 项目2、3和4中描述的粘附和细胞因子产生;以及 (b)膀胱器官结构由初级膀胱肌共 与移行上皮细胞一起培养以形成管状结构 在项目3中用作导管感染的体外模型。 (3)分子分型技术的应用，包括核糖体分型 以及检测限制性片段长度多态性的方法， 阴道和尿液E.杆菌分离株 从入组研究的患者中连续收集， 项目1。 (4)使用分子遗传技术，包括聚合酶链 用特异性DNA探针进行菌落印迹反应和杂交， 检测尿路致病性E.大肠杆菌收集的妇女有症状的尿路感染， 项目1为以前公认的大肠杆菌尿毒力决定因子。杆菌 以及项目3中新发现的毒力因子。 (5)维持现有的尿路致病性E.杆菌菌株 和乳杆菌分离物，并建立额外的 这些物种以及建立铜绿假单胞菌菌株库， 支持项目1、2、3和4。

The UTI Core Laboratory will provide necessary cell culture, microbiological and molecular typing procedures for all four projects in the Program. Centralized performance of these specialized procedures assures their availability, high quality, and reduced cost. The following services will be provided: (1) Microbiological procedures for the isolation and characterization of E. coli and Lactobacillus in Projects 1 and 3. (2) Culture of (a) primary transitional epithelial cells for use in studies of the regulation of glycosphingolipid synthesis, bacterial adherence, and cytokine production described in Projects 2, 3, and 4; and (b) bladder organ structures consisting of primary bladder muscle co- cultured with transitional epithelial cells to form tubularized structures to be used as an in vitro model for catheter infections in Project 3. (3) The application of molecular typing techniques, including ribotyping and methods of detecting restriction fragment length polymorphism, to test for genetic relatedness among vaginal and urinary E. coli isolates serially collected from patients enrolled in the studies described in Project 1. (4) Use of molecular genetic techniques, including polymerase chain reaction and hybridization of colony blots with specific DNA probes to test uropathogenic E. coli collected from women with symptomatic UTI in Project 1 for previously recognized urovirulence determinants of E. coli as well as newly identified virulence factors found in Project 3. (5) Maintenance of existing collections of uropathogenic E. coli strains and Lactobacillus isolates and establishment of additional collections of these species as well as establishment of a P. aeruginosa strains bank to support Projects 1, 2, 3, and 4.