MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA

极化上皮中基于微管的囊泡运输

基本信息

  • 批准号:
    6564275
  • 负责人:
  • 金额:
    $ 14.67万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2002
  • 资助国家:
    美国
  • 起止时间:
    2002-01-01 至 2002-12-31
  • 项目状态:
    已结题

项目摘要

In many eukaryotic cells, the subcellular positioning and dynamics of membranous organelles is dependent on microtubules and microtubule- based transport. The arrangement of the microtubule cytoskeleton along the apico-basal axis of simple polarized epithelia suggests that these filaments provide a structural framework that both yields stability to themonolayer and provides a substrate for transport ofmaterials across the cell. It appears that transcellular movement in the basal-to-apical direction, i.e. toward microtubule minus ends, is highly dependent on microtubules, suggesting tht the motor protein, cytoplasmic dynein, andits activator, dynactin are key players in this process. Polarized epithelia are therefore a useful model system for in-depth study of dynein-and dynactin-based intracellular motility. Understanding of mechanisms ofmicrotubule dynamics and microtubule- based motility has been significantly extended by the application of video-enhanced microscopy techniques, as they allow evaulation in real time of complex subcellular behaviors. Studies of intracellular membrane traffic and endomembrane dynamics have benefited equally fromthis sort of analysis. Inthis proposal, a series of experiments designed to elucidate the transcytotic pathway in WIF-B cells and to determine how cytoplasmic dynein and dynactin contribute to transcellular movement in WIF-B and MDCK are proposed. Membrane dynamics will be visualized using a novel surface labeling technique that allow traffic from the basal surface to the apical surface to be observed directly in real time by video-enhanced fluorescence microscopy. A separate line of investigation will explore the mechanism of microtubule nucleationin WIF-B and MDCK cells. Many epithelia are post-mitotic and their microtubule cytoskeletons lack a centralfocus, suggesting that conventional centrosomal nucleating mechanisms may not be involved. Sites for microtubule nucleationwill be identified and it will be determined if their centrosomes have the capacity to nucleate and release microtubules in vitro and in vivo.
在许多真核细胞中,亚细胞定位和动力学

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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TRINA A SCHROER其他文献

TRINA A SCHROER的其他文献

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{{ truncateString('TRINA A SCHROER', 18)}}的其他基金

Impact of a Disease-Associated Dynactin Variant on Motile Phenomena in Lung Epithelial Cells
疾病相关的 Dynactin 变体对肺上皮细胞运动现象的影响
  • 批准号:
    10704306
  • 财政年份:
    2022
  • 资助金额:
    $ 14.67万
  • 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
  • 批准号:
    8171285
  • 财政年份:
    2010
  • 资助金额:
    $ 14.67万
  • 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
  • 批准号:
    7957806
  • 财政年份:
    2009
  • 资助金额:
    $ 14.67万
  • 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
  • 批准号:
    7723664
  • 财政年份:
    2008
  • 资助金额:
    $ 14.67万
  • 项目类别:
Molecular mechanism of SIF formation by Salmonella Typhimurium
鼠伤寒沙门氏菌形成SIF的分子机制
  • 批准号:
    7197072
  • 财政年份:
    2007
  • 资助金额:
    $ 14.67万
  • 项目类别:
Molecular mechanism of SIF formation by Salmonella Typhimurium
鼠伤寒沙门氏菌形成SIF的分子机制
  • 批准号:
    7339653
  • 财政年份:
    2007
  • 资助金额:
    $ 14.67万
  • 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
  • 批准号:
    6410323
  • 财政年份:
    2001
  • 资助金额:
    $ 14.67万
  • 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
  • 批准号:
    6301128
  • 财政年份:
    2000
  • 资助金额:
    $ 14.67万
  • 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
  • 批准号:
    6105498
  • 财政年份:
    1999
  • 资助金额:
    $ 14.67万
  • 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
  • 批准号:
    6270727
  • 财政年份:
    1998
  • 资助金额:
    $ 14.67万
  • 项目类别:

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MDCK cell line evaluation
MDCK细胞系评估
  • 批准号:
    412774-2011
  • 财政年份:
    2011
  • 资助金额:
    $ 14.67万
  • 项目类别:
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Investigation the mechanism of renal stone formation by used the MDCK cell line.
利用MDCK细胞系研究肾结石形成的机制。
  • 批准号:
    11671547
  • 财政年份:
    1999
  • 资助金额:
    $ 14.67万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
MDCK CELL MUTANTS DEFECTIVE IN GLYCOPROTEIN MATURATION
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  • 批准号:
    3436819
  • 财政年份:
    1990
  • 资助金额:
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