MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
基本信息
- 批准号:6564275
- 负责人:
- 金额:$ 14.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-01-01 至 2002-12-31
- 项目状态:已结题
- 来源:
- 关键词:Golgi apparatus MDCK cell apical membrane basolateral membrane cellular polarity centrosome confocal scanning microscopy dynein ATPase electron microscopy endocytosis epithelium fluorescence microscopy fluorescent dye /probe intracellular transport light microscopy liver cells lysosomes membrane proteins microinjections microtubules protein transport transcytosis vesicle /vacuole video microscopy
项目摘要
In many eukaryotic cells, the subcellular positioning and dynamics of
membranous organelles is dependent on microtubules and microtubule-
based transport. The arrangement of the microtubule cytoskeleton
along the apico-basal axis of simple polarized epithelia suggests
that these filaments provide a structural framework that both yields
stability to themonolayer and provides a substrate for transport
ofmaterials across the cell. It appears that transcellular movement
in the basal-to-apical direction, i.e. toward microtubule minus ends,
is highly dependent on microtubules, suggesting tht the motor
protein, cytoplasmic dynein, andits activator, dynactin are key
players in this process. Polarized epithelia are therefore a useful
model system for in-depth study of dynein-and dynactin-based
intracellular motility.
Understanding of mechanisms ofmicrotubule dynamics and microtubule-
based motility has been significantly extended by the application of
video-enhanced microscopy techniques, as they allow evaulation in
real time of complex subcellular behaviors. Studies of intracellular
membrane traffic and endomembrane dynamics have benefited equally
fromthis sort of analysis. Inthis proposal, a series of experiments
designed to elucidate the transcytotic pathway in WIF-B cells and to
determine how cytoplasmic dynein and dynactin contribute to
transcellular movement in WIF-B and MDCK are proposed. Membrane
dynamics will be visualized using a novel surface labeling technique
that allow traffic from the basal surface to the apical surface to be
observed directly in real time by video-enhanced fluorescence
microscopy.
A separate line of investigation will explore the mechanism of
microtubule nucleationin WIF-B and MDCK cells. Many epithelia are
post-mitotic and their microtubule cytoskeletons lack a centralfocus,
suggesting that conventional centrosomal nucleating mechanisms may
not be involved. Sites for microtubule nucleationwill be identified
and it will be determined if their centrosomes have the capacity to
nucleate and release microtubules in vitro and in vivo.
在许多真核细胞中,亚细胞定位和动力学
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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TRINA A SCHROER其他文献
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{{ truncateString('TRINA A SCHROER', 18)}}的其他基金
Impact of a Disease-Associated Dynactin Variant on Motile Phenomena in Lung Epithelial Cells
疾病相关的 Dynactin 变体对肺上皮细胞运动现象的影响
- 批准号:
10704306 - 财政年份:2022
- 资助金额:
$ 14.67万 - 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
- 批准号:
8171285 - 财政年份:2010
- 资助金额:
$ 14.67万 - 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
- 批准号:
7957806 - 财政年份:2009
- 资助金额:
$ 14.67万 - 项目类别:
REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT
基于细胞质动力蛋白的囊泡运输的调节
- 批准号:
7723664 - 财政年份:2008
- 资助金额:
$ 14.67万 - 项目类别:
Molecular mechanism of SIF formation by Salmonella Typhimurium
鼠伤寒沙门氏菌形成SIF的分子机制
- 批准号:
7197072 - 财政年份:2007
- 资助金额:
$ 14.67万 - 项目类别:
Molecular mechanism of SIF formation by Salmonella Typhimurium
鼠伤寒沙门氏菌形成SIF的分子机制
- 批准号:
7339653 - 财政年份:2007
- 资助金额:
$ 14.67万 - 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
- 批准号:
6410323 - 财政年份:2001
- 资助金额:
$ 14.67万 - 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
- 批准号:
6301128 - 财政年份:2000
- 资助金额:
$ 14.67万 - 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
- 批准号:
6105498 - 财政年份:1999
- 资助金额:
$ 14.67万 - 项目类别:
MICROTUBULE BASED VESICLE TRANSPORT IN POLARIZED EPITHELIA
极化上皮中基于微管的囊泡运输
- 批准号:
6270727 - 财政年份:1998
- 资助金额:
$ 14.67万 - 项目类别:
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