CORE--ADVANCED IMAGING SUPPORT

核心——高级成像支持

• 批准号: 6564381
• 负责人: Michael Kashgarian
• 金额: $ 14.1万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564381
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; digital imaging; electron microscopy; fluorescence microscopy; histopathology; imaging /visualization /scanning; immunocytochemistry

Description: (Abstract of the application) The major goal of the imaging core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of protein interactions at the cellular and molecular level. A wide range of imaging techniques applicable to the study of membrane and cytoskeletal proteins in cells, tissues, whole organs are provided. In addition, the utilization of transgenic technology offers new opportunities to study the phenotypic changes at the integrated whole animal level. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. Paraffin section light microscopy, still the gold standard for the examination of structural phenotypic changes, has been expanded to include localization of specific proteins using immunohistochemistry and of specific mRNAs using in situ hybridization and in situ reverse transcription. Confocal microscopy can be applied to tissues where analyses of changes in intracellular ion concentration can be assessed at the tissue level using ion sensitive fluorochromes resulting in an expansion of the capabilities of video imaging techniques which are applied at the cellular level. The utilization of electron microscopic immunocytochemical techniques has been improved for greater accuracy in localization using immunogold and in situ hybridization techniques. The value of these techniques is enhanced by the availability of digital image analysis systems that permit quantitative morphometric measurements and three-dimensional reconstruction and visualization. These techniques are extremely useful in cellular pathobiology but are not easily accessible to investigators and research trainees without previous training or experience. In addition the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.

产品描述：（申请摘要）成像核心的主要目标是促进定量和定性成像技术在细胞和分子水平上分析蛋白质相互作用的使用。提供了广泛的成像技术，适用于研究细胞，组织，整个器官中的膜和细胞骨架蛋白。此外，转基因技术的应用为在整体动物水平上研究表型变化提供了新的机会。新技术现在可用于更高分辨率的特定靶蛋白的定位，以及允许重要的显微镜，其中动态变化可以识别。石蜡切片光学显微镜，仍然是检查结构表型变化的金标准，已扩大到包括使用免疫组化定位特定蛋白质和使用原位杂交和原位逆转录定位特定mRNA。共聚焦显微镜可以应用于组织，其中可以使用离子敏感荧光染料在组织水平上评估细胞内离子浓度变化的分析，从而扩展了应用于细胞水平的视频成像技术的能力。电子显微镜免疫细胞化学技术的利用率已得到提高，在使用免疫金和原位杂交技术的定位更准确。这些技术的价值是提高了数字图像分析系统，允许定量形态测量和三维重建和可视化的可用性。这些技术在细胞病理学中非常有用，但对于没有先前培训或经验的研究人员和研究受训人员来说并不容易获得。此外，选择适当的技术或技术组合需要解释经验和了解方法的局限性。成像核心将提供专业知识和援助，利用光，免疫荧光，共聚焦和电子显微镜，特别强调转基因模型的表型和病理分析，免疫细胞化学，重要的显微镜和图像分析。该设施将提供这些专业技术和应用这些技术所需的设备，并将成为该计划项目成员之间合作的焦点。具体而言，核心将提供必要的设施，服务和教育，以有效应用成像技术，为该计划项目的各个成员的个人研究目标量身定制。