CORE--MICROSCOPY AND IMAGING CENTER

核心——显微成像中心

• 批准号: 6725904
• 负责人: Michael Kashgarian
• 金额: $ 19.74万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6725904
• 关键词: bioimaging /biomedical imaging; biomedical facility; computer program /software; confocal scanning microscopy; digital imaging; electron microscopy; histochemistry /cytochemistry; imaging /visualization /scanning; immunofluorescence technique; in situ hybridization; morphometry; renal tubular transport; tissue /cell preparation; video microscopy

The major goal of the Cell Biology Core is to facilitate the use of quantitative qualitative imaging techniques and in the analysis of transport processes at the cellular and molecular level. The range of imaging techniques which are applicable to the study of cells, tissues, whole organs and in wild type, transgenic and mutant animals have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, uunbiased stereological morphometry, and vital microscopy. These techniques are extremely useful in cell biology and physiology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpetation and knowledge of the limitations of the method. The core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members.

细胞生物学核心的主要目标是促进定量定性成像技术的使用，并在细胞和分子水平上分析运输过程。适用于研究细胞、组织、整个器官以及野生型、转基因和突变动物的成像技术的范围已大大扩大。新技术现在可用于更高分辨率的特定靶蛋白的定位，以及允许重要的显微镜，其中动态变化可以识别。这些方法包括免疫细胞化学、共聚焦显微镜、电子显微镜、原位杂交、无偏体视形态学和活体显微镜。这些技术在细胞生物学和生理学中非常有用，但不容易被没有先前训练或经验的研究人员所使用。此外，选择适当的技术或技术组合需要口译经验和对口译局限性的了解。 法该核心将提供光学、免疫荧光、共聚焦和电子显微镜利用方面的专业知识和帮助，特别强调转基因模型的表型和病理分析、免疫细胞化学、活体显微镜和图像分析。该设施将提供这些专业技术和应用这些技术所需的设备，并将成为该计划项目成员之间合作的焦点。具体而言，该核心将提供必要的设施，服务和教育，以有效应用成像技术，为各成员的个人研究目标量身定制。