Core-B-Specialized imaging

专业成像核心的主要目标是促进定量和定性成像技术在细胞和分子水平的运输生理学分析中的使用。适用于研究细胞、组织、整个器官和完整生物体中的转运系统的成像技术的范围已经大大扩展。新技术现在可用于更高分辨率的特定目标定位 蛋白质，以及允许重要的显微镜，其中动态变化可以识别。这些技术包括免疫细胞化学、共聚焦显微镜、电子显微镜、原位杂交、形态测定、原子力显微镜和活体显微镜。这些技术在细胞生理学和生物学中非常有用，但对于没有先前训练或经验的研究人员来说并不容易获得。此外，选择适当的技术或技术组合需要有口译经验和对口译局限性的了解。 法成像核心将提供专业知识和援助，利用光，免疫荧光，共聚焦，原子力和电子显微镜，特别强调转基因模型的表型和病理分析，免疫细胞化学，重要的显微镜和图像分析。该设施将提供这些专业技术和应用这些技术所需的设备，并将成为该计划项目成员之间合作的焦点。具体而言，核心将提供必要的设施，服务和教育，以有效应用成像技术，为该计划项目的各个成员的个人研究目标量身定制。

The major goal of the Specialized Imaging Core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of transport physiology at the cellular and molecular level. The range of imaging techniques which are applicable to the study of transport systems in cells, tissues, whole organs and in the intact organism have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, morphometry, atomic force microscopy and vital microscopy. These techniques are extremely useful in cell physiology and biology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The Imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, atomic force and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.