Core-B-Specialized imaging

核心-B-专业成像

• 批准号: 7659696
• 负责人: Michael Kashgarian
• 金额: $ 7.36万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7659696
• 关键词: Animals; Area; Atomic Force Microscopy; Biological; Biology; Cell physiology; Cells; Cellular biology; Collaborations; Computers; Confocal Microscopy; Consultations; Data; Development; Education; Electron Microscopy; Electrons; Ensure; Equipment; Goals; Gold; Image; Image Analysis; Imagery; Imaging Techniques; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Immunohistochemistry; In Situ; In Situ Hybridization; Individual; Knock-out; Knowledge; Light; Measurement; Methods; Microscopic; Microscopy; Modeling; Molecular; Mutation; Organ; Organism; Paraffin; Pathologic; Physiology; Postdoctoral Fellow; Preparation; Proteins; Quality Control; Range; Reagent; Research; Research Personnel; Resolution; Resources; Reverse Transcription; Services; Specimen; Standards of Weights and Measures; Students; System; Techniques; Technology; Tissues; Training; Transgenic Model; Transgenic Organisms; experience; immunocytochemistry; improved; investigator training; light microscopy; member; morphometry; new technology; programs; reconstruction; transgene expression

The major goal of the Specialized Imaging Core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of transport physiology at the cellular and molecular level. The range of imaging techniques which are applicable to the study of transport systems in cells, tissues, whole organs and in the intact organism have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, morphometry, atomic force microscopy and vital microscopy. These techniques are extremely useful in cell physiology and biology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The Imaging core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, atomic force and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members of the program project.

专门成像核心的主要目标是促进在细胞和分子一级使用定量和定性成像技术来分析运输生理学。可应用于研究细胞、组织、整个器官和完整生物体内转运系统的成像技术的范围已经大大扩大。现在可以使用新技术来更好地解决特定目标的本地化 此外，我们还可以使用蛋白质，以及可以识别动态变化的重要显微镜。包括免疫细胞化学、共聚焦显微镜、电子显微镜、原位杂交、形态计量学、原子力显微镜和生命显微镜。这些技术在细胞生理学和生物学中非常有用，但在没有经过培训或经验的情况下，研究人员不容易获得这些技术。此外，选择适当的技术或技术组合需要有解释方面的经验和对 方法。成像核心将在利用光、免疫荧光、共聚焦、原子力和电子显微镜方面提供专门知识和援助，特别强调转基因模型的表型和病理分析、免疫细胞化学、生命显微镜和图像分析。该设施将使人们能够接触到这些专门技术以及应用这些技术所需的设备，并将成为方案项目成员之间合作的一个协调中心。具体地说，该核心将为有效应用成像技术提供必要的设施、服务和教育，这些技术是根据该计划项目不同成员的个人研究目标量身定做的。