CORE--FLUORESCENCE LABORATORY

核心--荧光实验室

• 批准号: 6650603
• 负责人: MARSHALL H MONTROSE
• 金额: $ 26.84万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6650603
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; confocal scanning microscopy; digital imaging; fluorescence microscopy; fluorimetry; image processing; method development

Description: This core will provide access to equipment, as well as training in proper usage, for quantitative determinations of fluorescence in living cells. Because some of this instrumentation is expensive and time-consuming to set up, the goal is to provide a common facility to support individual research projects. Two types of equipment in current use will be available in this core: a) A computer-controlled fluorometer for determination of fluorescence in populations of cells, either in suspension or in monolayers; and b) a microscope-based digital imaging system. Both types of equipment will be used by investigators after they have been trained by the Core Director. Investigators from all of the projects have need for these facilities in some form or other.

描述：该核心将提供设备通道，以及 正确使用的培训，用于荧光定量测定 在活细胞中。因为有些仪器很昂贵， 设置起来很耗时，目标是提供一个通用设施， 支持个人研究项目。目前有两种设备 在该核心中将可使用：a）计算机控制的荧光计 为了测定细胞群中的荧光， 悬浮液或单层;和B）基于显微镜的数字成像 系统这两种类型的设备将被调查人员使用后， 已接受核心总监的培训。所有的调查人员 这些项目需要某种形式的设施。