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Core--Tissue culture laboratory

核心--组织培养实验室

基本信息

批准号：
6583196
负责人：
Lynda F Bonewald
金额：
$ 21.52万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-04-01 至 2003-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6583196
关键词：
biomedical facility cell line laboratory mouse osteoblasts osteocytes tissue /cell culture tissue /cell preparation

项目摘要

A centralized tissue culture facility is more economical than individual investigators perform their own cell culture. The proposed core would be an expansion to accommodate this program project of a tissue culture core that has been in operation since 1980 in the Division of Endocrinology. Reasons for establishing and maintaining a common tissue culture core include the following: 1. Quality control of cell lines, culture techniques, and solutions 2. Bulk preparation of cells and media gives an economical advantage 3. Optimal use of time, equipment and supplies 4. Experienced technicians 5. Consistency between preparations A centralized tissue culture facility is the most efficient means for maintaining cell lines. The cell lines are catalogued and frozen as back-up stocks in liquid nitrogen tanks equipped with alarm systems to prevent loss of important cell lines. All cell lines are screened for mycoplasma detection twice per year. Purchase of fetal calf serum and media in bulk is certainly more cost-effective. Prescreening of 3 to 4 sources of serum for optimal cell growth before purchase is most efficiently accomplished in the core facility. Media and PBS can be prepared at 10, 20, or 30 liters at a time and then tested before use. Bulk ordering of plasticware is also more cost effective. The incubators and hoods are checked and maintained on a periodic basis at specific scheduled times.

一个集中的组织培养设施比单个研究人员进行自己的细胞培养更经济。拟议的核心将是一个扩展，以适应自1980年以来在内分泌科运作的组织培养核心项目。建立和维持一个共同的组织培养核心的原因包括：1。细胞系的质量控制、培养技术和解决方案细胞和培养基的大量制备具有经济优势。时间、设备和物资的最佳利用经验丰富的技术人员。集中的组织培养设备是维持细胞系最有效的手段。这些细胞系被分类并冷冻在配备警报系统的液氮罐中作为备用库存，以防止重要细胞系的损失。每年对所有细胞系进行两次支原体检测筛查。批量购买胎牛血清和培养基当然更具成本效益。在购买前对3到4个血清源进行最佳细胞生长的预筛选是在核心设施中最有效地完成的。培养基和PBS可以在10、20或30升的时间准备，然后在使用前进行测试。塑料制品的批量订购也更具成本效益。在特定的计划时间定期检查和维护孵化器和通风柜。