Core--Tissue culture laboratory

集中的组织培养设施比个体研究者自己进行细胞培养更经济。拟议的核心将是一个扩展，以适应自1980年以来一直在运行的内分泌科组织培养核心的计划项目。建立和维持一个共同的组织培养核心的原因包括以下几点：1。细胞系、培养技术和溶液的质量控制2.大量制备细胞和培养基具有经济优势3.最佳利用时间、设备和用品4.技术人员5名。制备之间的一致性集中的组织培养设施是维持细胞系的最有效手段。将细胞系分类并冷冻在配备有警报系统的液氮罐中作为备用储备，以防止重要细胞系的损失。每年对所有细胞系进行两次支原体检测筛查。大量购买胎牛血清和培养基肯定更具成本效益。在核心设施中最有效地完成购买前对3至4种血清来源进行预筛选以获得最佳细胞生长。培养基和PBS可以一次制备10、20或30升，然后在使用前进行测试。大量订购塑料器皿也更具成本效益。在特定的预定时间定期检查和维护培养箱和通风柜。

A centralized tissue culture facility is more economical than individual investigators perform their own cell culture. The proposed core would be an expansion to accommodate this program project of a tissue culture core that has been in operation since 1980 in the Division of Endocrinology. Reasons for establishing and maintaining a common tissue culture core include the following: 1. Quality control of cell lines, culture techniques, and solutions 2. Bulk preparation of cells and media gives an economical advantage 3. Optimal use of time, equipment and supplies 4. Experienced technicians 5. Consistency between preparations A centralized tissue culture facility is the most efficient means for maintaining cell lines. The cell lines are catalogued and frozen as back-up stocks in liquid nitrogen tanks equipped with alarm systems to prevent loss of important cell lines. All cell lines are screened for mycoplasma detection twice per year. Purchase of fetal calf serum and media in bulk is certainly more cost-effective. Prescreening of 3 to 4 sources of serum for optimal cell growth before purchase is most efficiently accomplished in the core facility. Media and PBS can be prepared at 10, 20, or 30 liters at a time and then tested before use. Bulk ordering of plasticware is also more cost effective. The incubators and hoods are checked and maintained on a periodic basis at specific scheduled times.