Investigating selection for antimicrobial resistance by non-antibiotic drugs in freshwater microbial communities

研究淡水微生物群落中非抗生素药物对抗菌药物耐药性的选择

• 批准号: 2270322
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2270322
• 关键词: Investigating; selection; antimicrobial; resistance; non

The first paper showing non-antibiotic drugs (NADs) can select for antimicrobial resistance (AMR) in human gut-associated strains has just been published, raising concerns for selection for AMR in vivo. However, NADs are also discharged into freshwater environments from waste water treatment plants, alongside antibiotics and other antimicrobial compounds. How these multiple pressures may interact to select for AMR in freshwater environments and how they impact microbial diversity is a completely novel research area, with the potential to influence policy at an international level and improve fundamental understanding of AMR evolution and aquatic microbial ecology. The student will master several microbiological techniques, including classical culturing methods and molecular approaches such as PCR, qPCR, knockout mutant generation and cloning. Computer coding and bioinformatics skills will be developed, enabling interrogation of next-generation sequencing data and design of automated pipelines. Students will gain experience in analytical chemistry and engaging with a range of stakeholders from the pharmaceutical and waste water industries, as well as policy makers and regulators.

第一篇显示非抗生素药物（NADs）可以在人类肠道相关菌株中选择抗微生物药物耐药性（AMR）的论文刚刚发表，这引起了人们对体内AMR选择的关注。然而，NADs也与抗生素和其他抗菌化合物一起从废水处理厂排放到淡水环境中。这些多重压力如何相互作用以选择淡水环境中的抗菌素耐药性，以及它们如何影响微生物多样性是一个全新的研究领域，有可能影响国际层面的政策，并提高对抗菌素耐药性进化和水生微生物生态学的基本理解。该学生将掌握多种微生物学技术，包括经典的培养方法和分子方法，如PCR、qPCR、基因敲除突变体的产生和克隆。计算机编码和生物信息学技能将得到发展，使下一代测序数据和设计自动化管道成为可能。学生将获得分析化学方面的经验，并与来自制药和废水行业的一系列利益相关者以及政策制定者和监管机构进行接触。