REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING

本申请中描述的研究的长期目标是 阐明急性暴露在间歇性低氧-复氧环境中如何影响 控制冠状动脉和肺血管功能的信号机制。 这个实验室已经阐明了许多方面的活性氧物种是如何 (ROS)和一氧化氮衍生物种(RNS)与信号系统相互作用 它调节控制血管收缩和松弛的过程，以及 组织呼吸过程，重点是这些机制如何 在氧分压变化引起的反应中起作用。它是 假设ROS和RNS在早期的修改中具有重要作用 急性暴露于间歇性低氧引起的血管功能异常。因此， 本项目的总体目标是阐明ROS和RNS在 接触铅引起的血管信号转导机制的初步改变 控制牛功能的间歇性低氧-复氧 冠状动脉和肺动脉。第一个目标是确定如何 间歇性缺氧-复氧改变信号转导机制 牛冠状动脉和肺动脉平滑肌收缩功能。 第二个目的是研究间歇性缺氧-复氧是如何改变的。 调节冠状动脉和肺组织的内皮衍生信号机制 大血管和微血管制剂中的动脉功能。第三 目的研究间歇性低氧-复氧如何改变氧气感觉。 调节冠状动脉和肺动脉功能的信号机制。 这些研究可以提供有关间歇性的基本信息 低氧导致血管功能的初始变化， 可能是相关血管疾病发病机制中的重要因素 患有睡眠呼吸暂停和相关疾病。

The long range goals of the studies described in this application are to elucidate how acute exposure to intermittent hypoxia-reoxygenation influences signaling mechanisms that control coronary and pulmonary vascular function. This laboratory has elucidated many aspects of how reactive oxygen species (ROS) and nitric oxide-derived species (RNS) interact with signaling systems which regulate processes that control vascular contraction and relaxation, and tissue respiratory processes, with an emphasis on how these mechanisms could function in responses elicited by changes in oxygen tension. It is hypothesized that ROS and RNS have important roles in the early modifications of vascular function caused by acute exposure to intermittent hypoxia. Thus, the overall goals of this project are to elucidate the roles of ROS and RNS in the initial alterations of vascular signaling mechanisms caused by exposure to intermittent hypoxia-reoxygenation that control the function of bovine coronary and pulmonary arteries. The first aim focuses on determining how intermittent hypoxia-reoxygenation alters signaling mechanisms that control bovine coronary and pulmonary arterial smooth muscle contractile function. The second aim examines how intermittent hypoxia-reoxygenation alters endothelium-derived signaling mechanisms that regulate coronary and pulmonary arterial function in large vessel and microvascular preparations. The third aim studies how intermittent hypoxia-reoxygenation alters oxygen sensing signaling mechanisms that regulate coronary and pulmonary arterial function. These studies could provide fundamental information on how intermittent hypoxia contributes to the initial alterations in vascular function which could be important factors in the pathogenesis of vascular diseases associated with sleep apnea and related disorders.