MULTIDISCIPLINARY PROGRAM IN FEMALE REPRODUCTION

女性生殖多学科计划

• 批准号: 6521060
• 负责人: Eugene D. Albrecht
• 金额: $ 93.81万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6521060
• 关键词: clinical research; cooperative study; female reproductive system; fertility; growth factor; hormone regulation /control mechanism; reproductive hormone

The objective of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Maryland Medical Center is to conduct innovative basic and clinical research in the reproductive sciences and to apply the knowledge gained from these studies to improve understanding of the etiology and treatment of infertility in women. A multidisciplinary experimental approach will be taken in this program to study function of the pituitary-ovarian- uterine axis as related to the central theme on cellular remodeling. In Project I (Karen A. Gregerson, PI), the physiological importance of an inwardly-rectifying, dopamine-activated pituitary K+ channel to prolactin secretion and fertility will be determined in transgenic mice in which the function of this novel membrane channel is interrupted by dominant-negative mutation. In Project II (Gerald J. Pepe, PI), the regulatory role of estrogen on fetal ovarian follicular and oocyte development and the impact on fertility will be assessed in baboons in which placental estrogen synthesis is suppressed in utero by administration of a novel aromatase inhibitor. In Project III (Robert D. Koos, PI), the physiological role of members of the fibroblast growth factor family, bFGF and keratinocyte growth factor, on ovarian and uterine function and fertility will be studied in transgenic mice in which the truncated, dominant-negative FGF receptors are spatially and temporally overexpressed. A combined clinical-basic research study is proposed in Project IV (Eugene D. Albrecht, PI), in which the roles of estrogen and progesterone, acting via vascular endothelial growth/ permeability factor and bFGF, on endometrial angiogenesis and fertility will be investigated in vivo in baboons and in vitro in the human endometrium. Each of the research projects will draw heavily upon the Cell Immunocytochemistry/In Situ Hybridization Core (Gloria E. Hoffman, PI) for the cellular localization of regulatory peptides and steroid hormone receptors. Integration of the scientific effort in this SCCPRR will be achieved through the thematic focus on cellular remodeling, common technological molecular approaches, and an established tradition of collaborative research.

专业合作社中心方案的目标是： 马里兰州医科大学生殖研究中心（SCCPRR） 中心是进行创新的基础和临床研究， 生殖科学和应用从这些方面获得的知识 研究，以提高对病因和治疗的理解， 女性不孕症。 一个多学科的实验方法将 参加这个项目是为了研究垂体卵巢的功能， 子宫轴与细胞重塑的中心主题有关。 在项目I（Karen A. Gregerson，PI）， 一个内向整流，多巴胺激活的垂体K+通道， 将在转基因小鼠中测定催乳素分泌和生育力 其中这种新的膜通道的功能被 显性阴性突变 在项目II（Gerald J. Pepe，PI）中， 雌激素对胎儿卵巢卵泡和卵母细胞的调节作用 将在狒狒中评估对生育力的影响， 胎盘雌激素的合成在子宫内被抑制， 给予新的芳香酶抑制剂。 在项目III（罗伯特 D. Koos，PI），成纤维细胞生长的生理作用成员 因子家族，bFGF和角质形成细胞生长因子，对卵巢和 将在转基因小鼠中研究子宫功能和生育能力， 截短的显性负性FGF受体在空间上和 暂时过度表达。 一项结合临床和基础研究的研究是 在项目IV中提出（尤金D. Albrecht，PI），其中的角色 雌激素和孕激素，通过血管内皮生长起作用/ 渗透因子和碱性成纤维细胞生长因子对子宫内膜血管生成和生育力的影响 将在狒狒体内和人体体外进行研究 子宫内膜 每个研究项目都将大量利用 细胞免疫细胞化学/原位杂交核心（格洛丽亚E.霍夫曼， PI）用于调节肽和类固醇的细胞定位 激素受体 在本SCCPRR中整合科学工作 将通过对细胞重塑的专题关注来实现， 共同的技术分子方法，以及既定的传统 合作研究。