CORE--HYBRIDOMA/CELL CULTURE/IMMUNE MONITORING

核心--杂交瘤/细胞培养/免疫监测

• 批准号: 6592795
• 负责人: HARRY D BEAR
• 金额: $ 4.12万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-16 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592795
• 关键词: antibody formation; biomedical facility; hybridomas; monoclonal antibody; tissue /cell culture

The Hybridoma/Cell Culture/Immune Monitoring Shared Resource of the MCC has been active since 1983. Initially, this core laboratory was established for the sole purpose of creating hybridomas and producing monoclonal antibodies for cancer research scientists at VCU. This technology has been critical for several different lines of investigation, including identification and purification of cancer-critical for several different lines of investigation, including identification of purification of cancer-related proteins and localization of these proteins and other molecules in cells and tissues. Over the past decade, as monoclonal antibodies and hybridoma cell lines have become more available from commercial sources, the need to create new hybridoma has decreased somewhat. Simultaneously, this shared resource has expanded its role to include production of monoclonal antibodies, large scale culture of cell lines other than hybridomas, growth of large numbers of lymphocytes for adoptive immunotherapy, mycoplasma testing of cell lines of all types, harvest and preparation of blood samples for correlative studies associated with clinical trials, and harvest and preparation of blood and lymphocyte samples for immunologic monitoring of endpoints associated with clinical immunotherapy trials. As with the original hybridoma production services, all of these functions serve cancer research investigators for whom it would be cumbersome and inefficient to establish the same capabilities in each of their separate laboratories. While preference is given to funded Cancer Center researchers, all VCU scientists are able to access the facility's services. Funding of this shared resource will be obtained from the CCSG and from a system of user fees designed to recover cost of supplies, while still providing a very cost- efficient service to research scientists at VCU and the Cancer Center.

MCC的杂交瘤/细胞培养/免疫监测共享资源自1983年以来一直活跃。最初，建立这个核心实验室的唯一目的是为VCU的癌症研究科学家创建杂交瘤和生产单抗。这项技术对几个不同的研究领域至关重要，包括癌症的鉴定和纯化--这对几个不同的研究领域至关重要，包括鉴定癌症相关蛋白质的纯化以及这些蛋白质和其他分子在细胞和组织中的定位。在过去的十年里，随着单抗和杂交瘤细胞系越来越多地从商业来源获得，创造新杂交瘤的需求有所减少。与此同时，这一共享资源的作用已扩大到包括生产单抗，大规模培养杂交瘤以外的细胞系，培养大量用于过继免疫治疗的淋巴细胞，对所有类型的细胞系进行支原体检测，采集和准备与临床试验相关的研究的血液样本，以及采集和准备血液和淋巴细胞样本，用于与临床免疫治疗试验相关的终点的免疫监测。与最初的杂交瘤生产服务一样，所有这些功能都是为癌症研究人员服务的，对他们来说，在每个单独的实验室建立相同的能力将是繁琐和低效的。虽然优先考虑资助癌症中心的研究人员，但所有VCU的科学家都能够使用该设施的服务。这一共享资源的资金将从CCSG和旨在收回供应成本的用户收费系统中获得，同时仍为VCU和癌症中心的研究科学家提供非常经济高效的服务。