CORE--TRANSGENIC MOUSE/EMBRYONIC STEM CELL

核心--转基因小鼠/胚胎干细胞

• 批准号: 6563713
• 负责人: Lutz Birnbaumer
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563713
• 关键词: biomedical facility; embryonic stem cell; genetically modified animals; laboratory mouse

Description: The UCLA Transgenic Mouse/Embryonic Stem (ES) Cell Shared Resource was established to accommodate individual investigator?s needs in the production of transgenic and knockout mice and to provide for rederivation of strains to create specific pathogen-free mice. Resource personnel serve as consultants to many different investigations providing information on specific transgenic animal models, the design and assembly of DNA constructs, and the development of new methods. Materials can also be provided to investigators who wish to perform projects in their own laboratories. There are two separate components of the resource: Transgenic Mouse and ES Cell Culture. The Transgenic Mouse Facility was established in 1991. Although not funded by NCI in the last competitive renewal application, the institution has subsidized the facility?s operation because access to transgenic and knockout technology plays a vital role in the Cancer Center?s research mission. The facility performs pronuclear injections of DNA and blastocyst injections of targeted ES cells, rederives mouse strains, and maintains transgenic animal lines in their initial stages. The Embryonic Stem Cell Facility is not yet operational. Institutional funds have been utilized to recruit a director, renovate space, and provide equipment for the facility. Once operational, a formal service for culture, electroporation, drug selection, and expansion of ES clones will be available. The measure of usage is based on injection days for the transgenic facility and targeting projects for the ES facility. The total capacity for the transgenic facility is 240 injection days and for the ES facility, 25 cell targeting projects. Twenty four UCLA faculty members used the resource representing assistance to 66 individual projects. Of these, use by peerreviewed Cancer Center members totaled 60 percent. The total operating budget of the shared resource for 1997-1998 is $259,672, with 25 percent requested, 50 percent to be obtained from AIDS chargebacks, and 25 percent from institutional support.

描述：UCLA转基因小鼠/胚胎干（ES）细胞共享 是否已建立资源以容纳个体研究者？的需要， 生产转基因和基因敲除小鼠，并提供 菌株来制造无特定病原体的小鼠。资源人员担任 为许多不同的调查提供咨询， 转基因动物模型，DNA构建体的设计和组装，以及 开发新方法。也可以向调查人员提供材料 他们希望在自己的实验室里完成项目。有两 资源的独立组成部分：转基因小鼠和ES细胞培养。 转基因小鼠设施成立于1991年。虽然没有得到资助， NCI在上一次竞争性续约申请中，该机构已 资助了这个机构因为获得转基因和基因敲除 技术在癌症中心发挥着至关重要的作用的研究使命。的 设施进行原核注射的DNA和胚泡注射， 靶向ES细胞，再衍生小鼠品系，并维持转基因动物 线在其初始阶段。 胚胎干细胞设施尚未投入使用。机构资金 已被用来招聘董事，翻新空间，并提供 设施的设备。一旦运作，一个正式的文化服务， 电穿孔、药物选择和ES克隆的扩增将是可用的。 使用率的测量是基于转基因设施的注射天数 并为ES设施定位项目。的总容量 转基因设施为240注射日，ES设施为25细胞 瞄准项目。24名加州大学洛杉矶分校的教职员工使用了 为66个项目提供援助。其中，通过同行评审 癌症中心的成员占60%。总业务预算 1997-1998年的共享资源为259，672美元，要求提供25%， 50%来自艾滋病退款，25%来自 机构支持。