3D RENDITION/QUANTITATIVE ANALYSIS GI2 DEFICIENT MICE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mice with a disrupted gene encoding (alpha subunit of Gi2 signaling protein) develop inflammatory bowel disease resembling human ulcerative colitis (UC; 1,2) and exhibit reduced number of intestinal Peyers patches. MRM imaging offers a unique opportunity to obtain relevant morphometric data. The changes are 100% penetrant, meaning they occur in all mutant animals, which makes the Gi2 mouse a good model for development and fine tuning of technical and computational refinements needed to make MRM an accessible tool to the scientific community. Problems preparing histological cuts from wild type mice in registry with those prepared from Gi2 mice make it very difficult to both qualitatively and quantitatively describe structural alterations in cerebellums of Gi2 mice. Insight is impossible to obtain using conventional serial histology without a major effort, innumerable manpower hours and manual recording of the data susceptible to errors associated with manual anno