3D RENDITION/QUANTITATIVE ANALYSIS GI2 DEFICIENT MICE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。基因编码中断(GI2信号蛋白的阿尔法亚单位)的小鼠会患上类似于人类溃疡性结肠炎(UC；1，2)的炎症性肠病，并表现出较少的肠道Peyers斑块。磁共振成像提供了一个独特的机会来获得相关的形态测量数据。这些变化是100%穿透的，这意味着它们发生在所有突变动物中，这使得GI2小鼠成为一个很好的模型，用于开发和微调所需的技术和计算改进，使MRM成为科学界可访问的工具。从野生型小鼠制备的组织切片与从GI2小鼠制备的组织切片相一致的问题使得很难定性和定量地描述GI2小鼠小脑的结构变化。使用传统的连续组织学是不可能获得洞察力的，除非付出大量的努力、无数的人力时间和手动记录易受与手动肛门相关的错误的数据

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mice with a disrupted gene encoding (alpha subunit of Gi2 signaling protein) develop inflammatory bowel disease resembling human ulcerative colitis (UC; 1,2) and exhibit reduced number of intestinal Peyers patches. MRM imaging offers a unique opportunity to obtain relevant morphometric data. The changes are 100% penetrant, meaning they occur in all mutant animals, which makes the Gi2 mouse a good model for development and fine tuning of technical and computational refinements needed to make MRM an accessible tool to the scientific community. Problems preparing histological cuts from wild type mice in registry with those prepared from Gi2 mice make it very difficult to both qualitatively and quantitatively describe structural alterations in cerebellums of Gi2 mice. Insight is impossible to obtain using conventional serial histology without a major effort, innumerable manpower hours and manual recording of the data susceptible to errors associated with manual anno