3D RENDITION/QUANTITATIVE ANALYSIS GI2 DEFICIENT MICE

3D 呈现/定量分析 GI2 缺陷小鼠

• 批准号: 7181529
• 负责人: Lutz Birnbaumer
• 金额: $ 2.13万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181529
• 关键词: bioimaging /biomedical imaging; biomedical resource; imaging /visualization /scanning; microscopy; technology /technique development

DESCRIPTION (provided by applicant): The enormous growth in the use of small animals for basic biomedical research has created a compelling need for new approaches to structural and functional phenotyping in the rodent. We propose continuation of the Duke Integrated Center for In Vivo Microscopy, an NIH/NCRR National Resource with an explicit focus on meeting this need. We propose four integrated cores that will provide researchers from around the world new image-based methods for structural and functional phenotyping using magnetic resonance microscopy (MRM). Core A: We will develop functional microscopy in the rodent to support cardiac motion analysis, pulmonary perfusion using arterial spin tagging and pulmonary ventilation using hypcrpolarized gas imaging. Core B: We will develop more efficient encoding strategies to increase our throughput for MR Histology by at least 500%. We will develop methods for actively staining fixed tissues for 3D MR histology. We will develop methods to measure regional neurodegeneration in the mouse brain. Core C: We will develop a new generation of technologies for physiologic support and monitoring of small animals permitting mutlimodality imaging (MRM. MicroPET, microCT, etc.) and image registration and long term studies on animals with compromised immunologic systems. Core D: We will develop visual informatics tools to permit effective archive, access, and analysis of very large multidimensional image sets from multiple modalities. The technologies will support a total of 16 collaborators anxious to use small animal imaging to extend the horizons of topics ranging from cancer to neurobiology. An aggressive education and dissemination program is included that will include undergraduate and graduate students, as well as post-doctoral fellows.

描述（由申请人提供）： 小动物用于基础生物医学研究的巨大增长迫切需要新的啮齿动物结构和功能表型分析方法。我们建议继续建立杜克体内显微镜综合中心，该中心是 NIH/NCRR 国家资源中心，明确致力于满足这一需求。我们提出了四个集成核心，将为世界各地的研究人员提供基于图像的新方法，使用磁共振显微镜（MRM）进行结构和功能表型分析。核心A：我们将开发啮齿动物的功能显微镜，以支持心脏运动分析、使用动脉自旋标记的肺灌注和使用超极化气体成像的肺通气。核心 B：我们将开发更高效的编码策略，将 MR 组织学的吞吐量提高至少 500%。我们将开发用于 3D MR 组织学的固定组织主动染色方法。我们将开发测量小鼠大脑区域神经变性的方法。核心 C：我们将开发新一代的小动物生理支持和监测技术，允许对免疫系统受损的动物进行多模态成像（MRM、MicroPET、microCT 等）以及图像配准和长期研究。核心D：我们将开发视觉信息学工具，以允许对来自多种模式的非常大的多维图像集进行有效的存档、访问和分析。这些技术将为总共 16 名合作者提供支持，他们渴望利用小动物成像来扩展从癌症到神经生物学等主题的视野。其中包括一项积极的教育和传播计划，其中包括本科生、研究生以及博士后研究员。