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Dissecting the role of root exudates in root density growth responses in plants

剖析根分泌物在植物根密度生长反应中的作用

基本信息

批准号：
2272100
负责人：
金额：
--
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2272100
关键词：
Dissecting role root exudates density

项目摘要

BackgroundPlants can detect the density of roots in the rhizosphere and use this information to determine the spatio-temporal allocation of root and shoot growth. The mechanisms by which plants detect and respond to root density remain unclear, but biochemical root exudates are likely to play an important role. Strigolactones are hormones that inhibit root and shoot system growth, but which are also exuded into the soil; they are therefore an excellent candidate signalling molecule for root density perception.Objectives1. Assess the role of strigolactones in root density perception and response.2. Identify novel exudates that act in plant communication in the rhizosphere.3. Understand the mechanisms by which plants respond to high root density.NoveltyAlthough plant communication in the rhizosphere is a long-recognized phenomenon, very little work has been undertaken to uncover the molecular signalling mechanisms that underlie it. This project thus represents a novel line of investigation into an important biological phenomenon. TimelinessInhibition of shoot growth by high root density in agricultural contexts likely acts as a major limit on crop yield. This project is thus highly timely, as understanding this phenomenon could unlock rapid increases in yield.Experimental ApproachTo assess the role of strigolactone in root density perception, we will use existing molecular genetic tools in the model plant Arabidopsis and in the crop species pea and barley, including strigolactone mutants and reporter lines. To identify new exudates that regulate plant-plant communication, we will collect exudates from barley and fractionate them, and use mass spectrometry to identify possible candidate signalling molecules. To understand the mechanisms by which plants to respond these signals, we will screen a barley diversity panel to perform QTL mapping for genes that regulate the response to root density. We will then identify and characterise the function of these genes.

背景植物可以探测根际的根密度，并利用这一信息来确定根和芽生长的时空分配。植物检测和响应根密度的机制尚不清楚，但生化根分泌物可能发挥重要作用。独脚金内酯是抑制根和芽系统生长的激素，但也会分泌到土壤中;因此，它们是根密度感知的优秀候选信号分子。评估独脚金内酯在根密度感知和响应中的作用.确定新的分泌物在植物根际通讯中的作用。了解植物对高根系密度的反应机制。新颖性虽然植物根际通讯是一个长期被认识的现象，但很少有人致力于揭示它背后的分子信号机制。因此，本项目代表了一个重要的生物学现象的新的研究方向。在农业环境中，高根系密度对新梢生长的抑制可能是作物产量的主要限制。因此，这个项目是非常及时的，因为了解这一现象可以解锁产量的快速增加。实验方法为了评估独脚金内酯在根密度感知中的作用，我们将使用现有的分子遗传工具在模式植物拟南芥和作物物种豌豆和大麦中，包括独脚金内酯突变体和报告系。为了确定新的分泌物，调节植物-植物通信，我们将收集大麦和fracrestimulating他们的分泌物，并使用质谱法来确定可能的候选信号分子。为了了解植物响应这些信号的机制，我们将筛选大麦多样性小组，对调节根密度响应的基因进行QTL定位。然后我们将识别和鉴定这些基因的功能。