A novel embryonic poly(A) binding protein

一种新型胚胎多聚腺苷酸结合蛋白

• 批准号: 6602242
• 负责人: Michael D Sheets
• 金额: $ 7.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6602242
• 关键词: Xenopus; antibody; cytoplasm; developmental genetics; immunoprecipitation; messenger RNA; polyadenylate; posttranscriptional RNA processing; protein protein interaction; protein structure function; radiotracer; transcription factor; translation factor; vertebrate embryology; yeast two hybrid system

DESCRIPTION (provided by applicant): Our ultimate goal is to understand the mechanisms by which maternally stored molecules control the earliest stages of vertebrate development. In particular, we are interested in the mechanisms that control the translational activation of stored maternal mRNAs during oocyte maturation and embryonic development prior to zygotic transcription. The translation of many stored maternal mRNAs is activated when their 3' poly (A) tails are elongated in a specific and developmentally regulated poly (A) addition reaction that takes place in the cytoplasm. This application is concerned with the regulated addition of poly (A) tails to maternal mRNAs that occurs during projesterone-induced oocyte maturation of the frog, Xenopus laevis. Specifically, we propose that a novel poly (A) tail binding protein that we have identified and named embryonic PABII (ePABII) is required for the selective 3' poly (A) elongation of particular maternal mRNAs during oocyte maturation. The developmental expression pattern of ePABII and its cytoplasmic location provide compelling circumstantial evidence that ePABII is involved in the dynamic mRNA poly (A) tail changes that occur during oocyte maturation and the maternal stages of development. This RO3 application describes several types of experiments designed to test directly the potential functional role of ePABII in maturation-specific mRNA poly (A) tail changes. Data from these initial experiments will provide the preliminary evidence critical for a more comprehensive RO1 grant that will focus on the mechanistic role of ePABII in the maternally controlled development of Xenopus laevis and other vertebrates.

描述（由申请人提供）：我们的最终目标是了解母体储存的分子控制脊椎动物发育最早阶段的机制。我们特别感兴趣的是在合子转录之前的卵母细胞成熟和胚胎发育过程中控制储存的母体 mRNA 翻译激活的机制。当许多储存的母体 mRNA 的 3' 聚 (A) 尾在细胞质中发生的特定且发育调节的聚 (A) 加成反应中延长时，它们的翻译就会被激活。该应用涉及在孕酮诱导的非洲爪蟾卵母细胞成熟过程中向母体 mRNA 中调节多聚 (A) 尾的添加。具体来说，我们提出，我们已经鉴定并命名为胚胎 PABII (ePABII) 的新型聚 (A) 尾结合蛋白是卵母细胞成熟过程中特定母体 mRNA 选择性 3' 聚 (A) 延伸所必需的。 ePABII 的发育表达模式及其细胞质位置提供了令人信服的间接证据，表明 ePABII 参与卵母细胞成熟和母体发育阶段发生的动态 mRNA 聚 (A) 尾变化。该 RO3 应用描述了几种类型的实验，旨在直接测试 ePABII 在成熟特异性 mRNA 聚 (A) 尾变化中的潜在功能作用。这些初步实验的数据将为更全面的 RO1 拨款提供至关重要的初步证据，该拨款将重点关注 ePABII 在非洲爪蟾和其他脊椎动物母体控制发育中的机制作用。