Control of Spemann's organizer

控制斯佩曼的组织者

• 批准号: 6708079
• 负责人: Michael D Sheets
• 金额: $ 25.77万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6708079
• 关键词: Xenopus; biological signal transduction; body regions; cell cell interaction; cell growth regulation; fibroblast growth factor; gene expression; genetic promoter element; genetic transcription; green fluorescent proteins; histogenesis; mesoderm; neurogenesis; protein biosynthesis; transfection; vertebrate embryology

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to make advances in our understanding of how signaling pathways and gene expression collaborate to control the earliest patterning events in the developing vertebrate embryo. Toward this end, we are combining molecular and embryological experiments to elucidate the mechanisms that control the formation of Spemann's organizer in the model organism Xenopus laevis. Spemann's organizer is a group of cells that collectively act as the central signaling center that establishes the vertebrate body plan by patterning the mesoderm and inducing neural tissue. The organizer influences the fate of surrounding cells by two mechanisms, the secretion of inducing proteins such as noggin and chordin and the regulation of morphogenesis and cell motility that involves direct cell-cell contacts. Therefore a defining molecular feature of organizer cells is the expression of specific signaling molecules such as chordin that help it perform its functions. In the absence of FGF signaling within the cells of the organizer, production of the inducing protein chordin is diminished, but the production of other organizer-specific molecules including noggin is not effected. In addition mesodermal patterning dictated by the organizer is disrupted, but the formation of most anterior neural structures in the embryo (head), which also require organizer function, is relatively normal. We hypothesize that FGF signaling within the organizer cells controls an important subset of the organizer's inducing functions at least in part through controlling organizer-specific transcription of chordin. We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer.

描述（由申请人提供）：本提案的长期目标是在我们对信号通路和基因表达如何协同控制发育中的脊椎动物胚胎中最早的模式事件的理解方面取得进展。为此，我们正在结合分子和胚胎学实验来阐明在模式生物非洲爪蟾中控制Spemann组织者形成的机制。Spemann的组织者是一组细胞，它们共同作为中央信号中心，通过塑造中胚层和诱导神经组织来建立脊椎动物的身体计划。组织者通过两种机制影响周围细胞的命运，一种是诱导蛋白（如noggin和chordin）的分泌，另一种是涉及细胞间直接接触的形态发生和细胞运动的调节。因此，组织者细胞的一个决定性的分子特征是表达特定的信号分子，如帮助其执行其功能的弦蛋白。在组织者细胞内缺乏FGF信号的情况下，FGF的产生