Control of Spemann's organizer

控制斯佩曼的组织者

• 批准号: 7285142
• 负责人: Michael D Sheets
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7285142
• 关键词: Xenopus; biological signal transduction; body regions; cell cell interaction; cell growth regulation; fibroblast growth factor; gene expression; genetic promoter element; genetic transcription; green fluorescent proteins; histogenesis; mesoderm; neurogenesis; protein biosynthesis; transfection; vertebrate embryology

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to make advances in our understanding of how signaling pathways and gene expression collaborate to control the earliest patterning events in the developing vertebrate embryo. Toward this end, we are combining molecular and embryological experiments to elucidate the mechanisms that control the formation of Spemann's organizer in the model organism Xenopus laevis. Spemann's organizer is a group of cells that collectively act as the central signaling center that establishes the vertebrate body plan by patterning the mesoderm and inducing neural tissue. The organizer influences the fate of surrounding cells by two mechanisms, the secretion of inducing proteins such as noggin and chordin and the regulation of morphogenesis and cell motility that involves direct cell-cell contacts. Therefore a defining molecular feature of organizer cells is the expression of specific signaling molecules such as chordin that help it perform its functions. In the absence of FGF signaling within the cells of the organizer, production of the inducing protein chordin is diminished, but the production of other organizer-specific molecules including noggin is not effected. In addition mesodermal patterning dictated by the organizer is disrupted, but the formation of most anterior neural structures in the embryo (head), which also require organizer function, is relatively normal. We hypothesize that FGF signaling within the organizer cells controls an important subset of the organizer's inducing functions at least in part through controlling organizer-specific transcription of chordin. We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer.

描述（由申请人提供）：该提案的长期目标是在我们了解信号通路和基因表达如何协作以控制发展中脊椎动物胚胎中最早的图案事件的过程中。为此，我们将分子和胚胎学实验结合在一起，以阐明控制Spemann组织者在模型生物体Xenopus laevis中形成的机制。 Spemann的组织者是一组细胞，共同充当中央信号转传中心，通过对中胚层进行构图并诱导神经组织来建立脊椎动物的计划。组织者通过两种机制来影响周围细胞的命运，即诱导蛋白质（例如Noggin和Chordin）的分泌以及涉及直接细胞细胞接触的形态发生和细胞运动的调节。因此，组织者细胞的定义分子特征是特定信号分子（例如Chordin）的表达，可以帮助其执行其功能。在组织器细胞内没有FGF信号的情况下，产生 诱导蛋白质果蛋白的诱导减少，但没有影响包括Noggin在内的其他组织者特异性分子的产生。另外，由组织者决定的中胚层模式被破坏，但是胚胎中大多数前神经结构的形成（头）也需要组织者功能，这是相对正常的。我们假设组织器细胞中的FGF信号传导至少部分通过控制Chordin的组织者特异性转录来控制组织者诱导功能的重要子集。我们将通过研究组织者中FGF信号通路的作用以及Chordin在与Spemann组织者的最终诱导功能时的转录来解决我们的观点。