Quantitation Of Biomarkers Of Oxidative Stress By Nici-m

1996年，在NIEHS举办了关于测量人类氧化应激的讲习班。有一个普遍的共识是，开展一项研究，比较从同一样本测量的氧化应激的各种标记物，将是有价值的。其中一个这样的标记是F2a-异前列腺素，它通过NICI-MS进行定量。异前列腺素是一种类似前列腺素的化合物，它是由膜磷脂中花生四烯酸的化学氧化而不是酶催化氧化产生的。异前列腺素已被证明具有强大的生物活性，许多研究表明，它们是氧化应激动物模型中脂质过氧化的准确标记物。8-iso-F2a-isprostane是大多数研究中定量的特异性异构体。质谱学工作组被要求为NIEHS提供8-iso-F2a-异前列腺素分析，作为各种拟议生物标志物循环研究的一部分，并向NIEHS的科学家提供这一能力。

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. Isoprostanes are prostaglandin-like compounds that are produced as a result of the chemical rather than enzymatic oxidation of arachidonic acid in membrane phospholipids. The isoprostanes have been shown to possess potent biological activity, and a number of studies have shown them to be accurate markers of lipid peroxidation in animal models of oxidative stress. The 8-iso-F2a-isoprostane has been the specific isomer quantified in most studies. The Mass Spectrometry Workgroup was asked to provide 8-iso-F2a-isoprostane analyses for NIEHS as part of the round-robin study of the various proposed biomarkers as well as to provide this capability to NIEHS scientists.