Quantitation Of Biomarkers Of Oxidative Stress By Nici-m

1996年，在NIEHS举行了一次关于人体氧化应激测量的研讨会。人们普遍认为，开展一项比较从同一样本中测量的各种氧化应激标志物的研究是有价值的。其中一个这样的标记是f2a -异前列腺素，它是用NICI-MS定量的。异前列腺素是一种类似前列腺素的化合物，是由膜磷脂中的花生四烯酸的化学氧化而不是酶氧化产生的。异前列腺素已被证明具有强大的生物活性，许多研究表明它们是氧化应激动物模型中脂质过氧化的准确标记物。在大多数研究中，8-iso- f2a -异前列腺素是定量的特异性异构体。质谱工作组被要求为NIEHS提供8-iso- f2a -异前列腺素分析，作为各种生物标志物循环研究的一部分，并向NIEHS科学家提供这种能力。

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. Isoprostanes are prostaglandin-like compounds that are produced as a result of the chemical rather than enzymatic oxidation of arachidonic acid in membrane phospholipids. The isoprostanes have been shown to possess potent biological activity, and a number of studies have shown them to be accurate markers of lipid peroxidation in animal models of oxidative stress. The 8-iso-F2a-isoprostane has been the specific isomer quantified in most studies. The Mass Spectrometry Workgroup was asked to provide 8-iso-F2a-isoprostane analyses for NIEHS as part of the round-robin study of the various proposed biomarkers as well as to provide this capability to NIEHS scientists.