Quantitation Of Biomarkers Of Oxidative Stress By Nici-m

Nici-m 定量氧化应激生物标志物

• 批准号: 6677451
• 负责人: Kenneth Tomer
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6677451
• 关键词: alcoholic beverage consumption; antioxidants; biomarker; carcinogenesis; carotenoids; clinical research; eicosanoids; human data; human tissue; lipid peroxides; mass spectrometry; nutrition aspect of cancer; nutrition related tag; oxidative stress; peroxidation; prostaglandin analogs; tissue /cell culture

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. Isoprostanes are prostaglandin-like compounds that are produced as a result of the chemical rather than enzymatic oxidation of arachidonic acid in membrane phospholipids. The isoprostanes have been shown to possess potent biological activity, and a number of studies have shown them to be accurate markers of lipid peroxidation in animal models of oxidative stress. The 8-iso-F2a-isoprostane has been the specific isomer quantified in most studies. The Mass Spectrometry Workgroup was asked to provide 8-iso-F2a-isoprostane analyses for NIEHS as part of the round-robin study of the various proposed biomarkers as well as to provide this capability to NIEHS scientists.

1996年，在NIEHS举办了一个关于测量人类氧化应激的讲习班。人们普遍认为，开展一项比较从同一样本中测量的各种氧化应激标志物的研究是有价值的。一种这样的标志物是F2 a-异前列烷，其通过NICI-MS定量。异前列烷是类胡萝卜素化合物，其是由于膜磷脂中花生四烯酸的化学氧化而不是酶促氧化而产生的。异前列烷已被证明具有有效的生物活性，许多研究表明它们是氧化应激动物模型中脂质过氧化的准确标志物。8-iso-F2 a-异前列烷是大多数研究中定量的特定异构体。质谱工作组被要求为NIEHS提供8-iso-F2 a-异前列烷分析，作为各种拟议生物标志物的循环研究的一部分，并为NIEHS科学家提供这种能力。