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Protein Microcharacterization Facility

Protein Microcharacterization Facility
蛋白质微观表征设备
批准号：
7330676
负责人：
Kenneth Tomer
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7330676
关键词：
ProteinMicrocharacterizationFacility

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中文摘要
翻译
LSB的质谱工作组内建立了一个基于质谱的蛋白质微表征设施。该设施的目的是提供最先进的蛋白质质谱表征。这包括通过凝胶电泳分离的蛋白质，从表达系统中获得的蛋白质，从免疫沉淀实验中分离的蛋白质，以及翻译后修饰和加合物的鉴定。该设施还提供基于MS的蛋白质组学鉴定2-D凝胶电泳与其他团体合作。此外，PMCF已开始每月举办一次用户论坛，并就开放获取MALDI/TOF仪器的使用进行培训。
英文摘要
A mass spectrometry-based Protein Microcharacterization Facility has been established within the Mass Spectrometry Workgroup in LSB. The purpose of the Facility is to provide state-of-the-art mass spectrometric characterization of proteins. This includes proteins separated by gel electrophoresis, obtained from expression systems, isolated from immunoprecipitation experiments, and identification of posttranslational modifications and adducts. The Facility also provides MS-based proteomics identifications from 2-D gel electrophoresis in collaboration with other groups. Additionally, the PMCF has begun a monthly User's Forum and training on use of open access MALDI/TOF instrumentation.
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